{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:00:35Z","timestamp":1791205235404,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,12,12]],"date-time":"2012-12-12T00:00:00Z","timestamp":1355270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>The physiological behavior of <jats:italic>Helicobacter pylori<\/jats:italic> in different growth conditions was investigated to approach its growth standardization. <jats:italic>H. pylori<\/jats:italic> free\u2010living and biofilm modes of growth were assessed in four different liquid culture media (Brucella broth, brain heart infusion broth and Ham's F\u201012 supplemented with 2% fetal calf serum and Ham's F\u201012 without serum). Free\u2010living growth was monitored during 72\u00a0h in each medium and characterized for bacterial density, culturability, viability and morphology. The biofilm formation in the same media was evaluated for biomass production, colony forming unit (CFU) counts and microscopic visualization. Afterward, using Ham's F\u201012, the effect of amoxicillin and clarithromycin at sub\u2010 minimum inhibitory concentrations (sub\u2010MICs) was evaluated on <jats:italic>H. pylori<\/jats:italic> biofilm formation and <jats:italic>luxS<\/jats:italic> gene expression. Differences in the free\u2010living growth were observed between the media supplemented with serum and Ham's F\u201012 without serum. Biofilm formation was significantly dependent on the growth media used. Ham's F\u201012 seems to be a good medium to support both growth phenotypes of <jats:italic>H. pylori<\/jats:italic>. Moreover, sub\u2010MICs of antibiotics increased the biofilm formation and affected the <jats:italic>luxS<\/jats:italic> gene expression. Optimizing the growth conditions of <jats:italic>H. pylori<\/jats:italic>, especially in the biofilm mode, will be helpful to perform more accurate in\u2010depth studies that will allow increasing the knowledge about <jats:italic>H. pylori<\/jats:italic> biofilm, which should be a target to eradicate resistant infection.<\/jats:p>","DOI":"10.1111\/apm.12020","type":"journal-article","created":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T17:20:37Z","timestamp":1355419237000},"page":"549-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Helicobacter pylori<\/i> free\u2010living and biofilm modes of growth: behavior in response to different culture media"],"prefix":"10.1111","volume":"121","author":[{"given":"Lucinda J.","family":"Bessa","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Grande","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donato DI","family":"Iorio","sequence":"additional","affiliation":[{"name":"Department of Medical Oral Science and Biotechnology University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara DI","family":"Giulio","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela DI","family":"Campli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigina","family":"Cellini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u2018G. d'Annunzio\u2019 \u2013 Chieti\u2010Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01918.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i35.3971"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2000.00699.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.11.015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002849900344"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0567-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-009-9534-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1984-1987.2000"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3842-3850.2001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1128\/jcm.29.12.2835-2837.1991","article-title":"Evaluation of liquid media for growth of Helicobacter pylori","volume":"29","author":"Shahamat M","year":"1991","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2012.05165.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00481.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.10.3124-3132.2004"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01958-07"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-197"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-009-9558-9"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1479050505001845"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gassur.2005.10.019"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520701675965"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.03029.x"},{"key":"e_1_2_6_22_1","first-page":"467","article-title":"Helicobacter pylori isolates from proximal and distal stomach of patients never treated and already treated show genetic variability and discordant antibiotic resistance","volume":"23","author":"Marzio L","year":"2011","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2011.00910.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04911.x"},{"key":"e_1_2_6_25_1","volume-title":"Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; 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