{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:23:11Z","timestamp":1791181391412,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T00:00:00Z","timestamp":1352332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>Enteropathogenic<jats:italic>Escherichia coli<\/jats:italic>(<jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>) is usually associated with outbreaks and sporadic cases of severe infantile diarrhoea in the developing world, and less commonly with sporadic cases in developed countries. Very little evidence indicates that<jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>is a food\u2010borne pathogen for adults. In a previous study, two groups of adult travellers became ill, and<jats:italic>eae<\/jats:italic><jats:sup>+<\/jats:sup><jats:italic>E. coli<\/jats:italic>of serogroup O111 was isolated from affected individuals and epidemiologically linked to food consumption. Here the strain responsible was further investigated and characterized as an unusual atypical<jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>analysis of the designated type isolate showed the presence of the<jats:italic>rorf1<\/jats:italic>and<jats:italic>espB<\/jats:italic>genes of the<jats:styled-content style=\"fixed-case\">LEE<\/jats:styled-content>pathogenicity island, which was inserted at the chromosomal<jats:italic>selC<\/jats:italic>locus. The isolate was negative for the enteroaggregative<jats:italic>E. coli<\/jats:italic><jats:styled-content style=\"fixed-case\">EAST<\/jats:styled-content>\u20101 toxin present in other strains of<jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>associated with food\u2010borne outbreaks. The strain adhered sparsely to<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>p\u20102 cell monolayers in a diffuse manner, but fluorescent actin staining demonstrated that it was capable of inducing polymerization of actin at the sites of bacterial attachment. Strain P2583 is the first<jats:styled-content style=\"fixed-case\">EAST<\/jats:styled-content>\u2010negative<jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>to be confirmed as a cause of outbreaks of infection in adults following the consumption of contaminated food or water.<\/jats:p>","DOI":"10.1111\/apm.12024","type":"journal-article","created":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T07:21:17Z","timestamp":1352359277000},"page":"494-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of a novel<scp>EAST<\/scp>\u2010negative enteropathogenic<i>E. coli<\/i>strain implicated in a food\u2010borne outbreak of diarrhoea in adults"],"prefix":"10.1111","volume":"121","author":[{"given":"Amy L.","family":"Wedley,","sequence":"first","affiliation":[{"name":"School of Medical Sciences University of Bradford Bradford 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