{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:50:33Z","timestamp":1759776633027},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T00:00:00Z","timestamp":1365465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>The most common way for cervical cancer to spread is through the lymphatic system. Tumor\u2010associated lymphatic endothelial cell (<jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content>) has been considered to play a crucial role in metastasis of certain cancers. The aim of this study was to isolate <jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content> from human cervical cancers and explore its involvement in metastasis\u2010associated behaviors <jats:italic>in vitro<\/jats:italic>. Lymphatic vessels in 62 cervix tissue specimens ranging from cervical intraepithelial neoplasia (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>) to advanced invasive cancer were detected using immunochemical staining with D2\u201040 antibody. Relation of lymphatic vessel density (<jats:styled-content style=\"fixed-case\">LVD<\/jats:styled-content>) to clinicopathological characters was analyzed. Primary <jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content>s were isolated by <jats:styled-content style=\"fixed-case\">LYVE<\/jats:styled-content>\u20101 immuno\u2010magnetic beads from cervical cancer tissues and verified through expression of <jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content> markers Prox\u20101 and D2\u201040, and then cultured <jats:italic>in vitro<\/jats:italic>. Invasiveness and viability of cells were assessed by transwell assay and typan blue exclusion, respectively. Our results showed that higher <jats:styled-content style=\"fixed-case\">LVD<\/jats:styled-content> was significantly associated with advanced <jats:styled-content style=\"fixed-case\">FIGO<\/jats:styled-content> stage, pelvic lymphatic nodal metastasis (<jats:styled-content style=\"fixed-case\">LNM<\/jats:styled-content>), and poorer cell differentiation. <jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content>s were successfully primarily isolated and cultured <jats:italic>in vitro<\/jats:italic>. Supernatant of <jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content> enhanced invasiveness of Hela cell, but did not significantly affect cell viability. In conclusion, <jats:styled-content style=\"fixed-case\">TLEC<\/jats:styled-content>s might actively promote lymphatic metastasis of cervical cancer. Further studies are needed to demonstrate the underlying mechanisms.<\/jats:p>","DOI":"10.1111\/apm.12068","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T09:23:40Z","timestamp":1365499420000},"page":"1162-1168","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Tumor\u2010associated lymphatic endothelial cell promotes invasion of cervical cancer cells"],"prefix":"10.1111","volume":"121","author":[{"given":"Liqiong","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouhua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan 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