{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T04:18:04Z","timestamp":1754194684090},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:00:00Z","timestamp":1366243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>Most tumors contain a minor population of cancer stem cells that are responsible for tumor heterogeneity, resistance to therapy and recurrence. Oct\u20104 is a transcription factor responsible for self\u2010renewal of stem cells, whereas the Notch family of receptors and ligands may play a pivotal role in the regulation of stem cell maintenance and differentiation. This study aimed at an evaluation of Oct\u20104 and Notch\u20101 expression in both carcinoma and stromal cells of 83 cases of primary bladder carcinoma and to study the relationship between them. Notch\u20101 was expressed in carcinoma and stromal cells of all malignant cases, where expression in both cell types was correlated with parameters indicating differentiation, such as low grade (p\u00a0&lt;\u00a00.05) and less proliferation (p\u00a0&lt;\u00a00.05). However, Notch\u20101 expression in stromal cells was associated with nodal metastasis (p\u00a0=\u00a00.016) and advanced stage (p\u00a0=\u00a00.030). 56.6 and 75.9% of carcinoma and stromal cells of malignant cases showed Oct\u20104 expression, respectively. Oct\u20104 expression in carcinoma cells or stromal cells was associated with aggressive features of bladder carcinoma, such as poor differentiation (p\u00a0=\u00a00.001), high proliferation (p\u00a0&lt;\u00a00.001, 0.030), and liability for recurrence (p\u00a0=\u00a00.010, p\u00a0&lt;\u00a00.001). There was an inverse relationship between Notch\u20101 and Oct\u20104 expression in carcinoma cells (p\u00a0=\u00a00.002), but stromal expression of Notch\u20101 was found to be associated with a nuclear pattern of Oct\u20104 expression in carcinoma cells (p\u00a0=\u00a00.030). Oct\u20104 as a stem cell marker is expressed in carcinoma cells and in stromal cells of bladder carcinoma, where they may cooperate in the progression of bladder carcinoma by acquiring aggressive features, such as a liability for recurrence and dissemination. Notch\u20101 is also expressed in both carcinoma cells and stromal cells of bladder carcinoma. Although they could share in enhancing differentiation, stromal expression of Notch\u20101 may have a bad impact, possibly through up\u2010regulation of the active nuclear form of Oct\u20104 in carcinoma cells.<\/jats:p>","DOI":"10.1111\/apm.12086","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T10:33:08Z","timestamp":1366281188000},"page":"982-996","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunohistochemical analysis of the role and relationship between Notch\u20101 and Oct\u20104 expression in urinary bladder carcinoma"],"prefix":"10.1111","volume":"121","author":[{"given":"Asmaa Gaber","family":"Abdou","sequence":"first","affiliation":[{"name":"Pathology Department Faculty of Medicine Menofiya University Shebein Elkom Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moshira Mohammed 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