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Ion concentrations in the <jats:styled-content style=\"fixed-case\">ASL<\/jats:styled-content> are determined by the cystic fibrosis transmembrane conductance regulator (<jats:styled-content style=\"fixed-case\">CFTR<\/jats:styled-content>) and the epithelial Na<jats:sup>+<\/jats:sup> channel (<jats:styled-content style=\"fixed-case\">ENaC<\/jats:styled-content>). Neonatal sepsis is a major risk factor for subsequent pulmonary disease in preterm newborns. Predominating are coagulase\u2010negative staphylococci (e.g., <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>taphylococccus epidermidis<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>taphylococccus aureus<\/jats:italic>). The aim of this study was to investigate modulation of CFTR, ENaC, mucins, proinflammatory cytokines, and inducible nitric oxide synthase (<jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content>) in respiratory epithelial cells after <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. epidermidis<\/jats:italic> 94B080 and <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic> 90B083 exposure. Bronchial epithelial cells were incubated with <jats:italic>S. epidermidis<\/jats:italic> 94B080 and <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic> 90B083 (neonatal blood isolates) for 1\u201336\u00a0h. Expression of CFTR, ENaC, <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content>, and mucins was analyzed by real\u2010time PCR and Western blotting. Release of cytokines was analyzed by ELISA, and production of NO by the Griess assay. Expression of CFTR significantly decreased after 36\u00a0h incubation with <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. epidermidis<\/jats:italic> and more prominently with <jats:italic>S. aureus<\/jats:italic>, whereas <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. epidermidis<\/jats:italic> caused a significant increase in the expression of \u03b2\u2010 and \u03b3\u2010ENaC. Expression of <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content> increased, but NO was not detected. Both staphylococci caused a decrease in the intracellular <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> concentration. <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic> induced increased secretion of IL\u20106, IL\u20108, and transforming nuclear factor (TNF)\u2010\u03b1 in a time\u2010dependent manner as compared with <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. epidermidis<\/jats:italic>. In conclusion, expression of ENaC, CFTR, and <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content> is modulated by exposure to <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. <jats:italic>aureus<\/jats:italic> 90B083 and <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. <jats:italic>epidermidis<\/jats:italic> 94B080. <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> is more potent in causing release of IL\u20106, IL\u20108, and TNF\u2010\u03b1 by bronchial epithelial cells as compared with <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. epidermidis<\/jats:italic>. The <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression for the mucus proteins <jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">MUC5AC<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">MUC5B<\/jats:styled-content> could not be measured, neither in the presence nor in the absence of bacteria.<\/jats:p>","DOI":"10.1111\/apm.12138","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T19:31:31Z","timestamp":1374694291000},"page":"814-826","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Modulation of ENaC, <scp>CFTR<\/scp>, and <scp>iNOS<\/scp> expression in bronchial epithelial cells after stimulation with <i><scp>S<\/scp>taphylococcus epidermidis<\/i> (94B080) and <i><scp>S<\/scp>taphylococcus aureus<\/i> 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