{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:01:39Z","timestamp":1792710099539,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T00:00:00Z","timestamp":1374624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>The present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. Subgingival biofilm samples were collected from the four deepest pockets (&gt;5\u00a0mm) of 17 patients with aggressive periodontitis (AgP) and 33 patients with chronic periodontitis (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>), first by paper point and thereafter by curette. Samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiological analysis by a commercially available assay (<jats:styled-content style=\"fixed-case\">IAI<\/jats:styled-content> Pado Test 4.5) that estimates total bacterial load and levels of <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>orphyromonas gingivalis<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>annerella forsythia<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reponema denticola<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>ggregatibacter actinomycetemcomitans<\/jats:italic>. Data analysis included frequency of detection, quantification and correlation of detection levels between the two sampling methods. <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>. gingivalis<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>. forsythia<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>. denticola<\/jats:italic> were detected in &gt;90% of the samples, and their detection levels exhibited a strong correlation between sampling methods. The detection consistency of <jats:italic>A. actinomycetemcomitans<\/jats:italic> was 56% between the two sampling methods. <jats:italic>A. actinomycetemcomitans<\/jats:italic> was more readily detected by paper point compared with curette collection with a stronger correlation between the two methods in AgP. Subgingival biofilm sampling by curette or paper point does not yield differences in the detection of the three \u2018red complex\u2019 species. However, <jats:italic>A. actinomycetemcomitans<\/jats:italic> was more consistently detected by means of paper point collection, which can be crucial in the decision to administer antibiotics as an adjunctive periodontal treatment.<\/jats:p>","DOI":"10.1111\/apm.12151","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T19:31:49Z","timestamp":1374694309000},"page":"347-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette"],"prefix":"10.1111","volume":"122","author":[{"given":"Georgios N.","family":"Belibasakis","sequence":"first","affiliation":[{"name":"Oral Microbiology and Immunology Faculty of Medicine Institute of Oral Biology Center of Dental Medicine University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Schmidlin","sequence":"additional","affiliation":[{"name":"Periodontology Clinic for Preventive Dentistry Periodontology and Cariology Center of Dental Medicine Faculty of Medicine University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Sahrmann","sequence":"additional","affiliation":[{"name":"Periodontology Clinic for Preventive Dentistry Periodontology and Cariology Center of Dental Medicine Faculty of Medicine University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1988.tb01598.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01260.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.11.49"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2000.035002108.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00004.x"},{"key":"e_1_2_6_8_1","first-page":"82","article-title":"Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment","volume":"1999","author":"Slots J","year":"2000","journal-title":"Periodontology"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060218"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100565"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1997.2.1.138"},{"key":"e_1_2_6_12_1","first-page":"38","article-title":"Molecular microbial diagnosis","volume":"2009","author":"Paster BJ","year":"2000","journal-title":"Periodontology"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016350903514848"},{"key":"e_1_2_6_14_1","first-page":"53","article-title":"Implementation of transgingival antibacterial photodynamic therapy (PDT) supplementary to scaling and root planing. 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