{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T03:20:42Z","timestamp":1768965642459,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T00:00:00Z","timestamp":1387756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"This publication is part of project \u201eWrovasc \u2013 Integrated Cardiovascular Centre\u201d, co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013 realized in Regional Specialist Hospital, Research and Development Centre in Wroclaw. European Funds \u2013 for the development of an innovative economy. We invest in your future.","award":["POIG.01.01.02-02-001\/08-00"],"award-info":[{"award-number":["POIG.01.01.02-02-001\/08-00"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Nogo\u2010B (Reticulon 4B) is considered to be a novel vascular marker, which may have a protective role in injury\u2010induced neointima formation and atherosclerosis. Nogo A\/B is found to be crucial for monocyte\/macrophage recruitment in acute inflammation and it is expressed in CD68\u00a0+\u00a0macrophages. We hypothesize that macrophage infiltration in atherosclerosis is not dependent on Nogo\u2010B expression in arterial wall. We have assessed Nogo\u2010B expression and macrophage accumulation in the iliac arteries of healthy organ donors and organ donors with cardiovascular risk factors. Paraffin sections of 66 iliac arteries, from 44 deceased organ donors (17 women and 27 men), were studied. The healthy and cardiovascular risk (<jats:styled-content style=\"fixed-case\">CVR<\/jats:styled-content>) subgroups were created. With regard to staging of the atherosclerotic process, the thickness of arterial intima was measured in digitalized images of H+E stained tissue sections. Immunohistochemical reactions (Nogo\u2010B and CD68) were carried out in all arteries (66 samples). Western blotting (WB\u201019 samples) and real\u2010time PCR (27 samples) were performed on selected arteries. Significantly higher Nogo\u2010B expression was demonstrated in the intima of the healthy subjects' subgroup, using immunohistochemistry. <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> and real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> revealed a trend toward lower Nogo\u2010B expression in the adventitia of the <jats:styled-content style=\"fixed-case\">CVR<\/jats:styled-content> subgroup. Furthermore, the thickness of the intima was found to negatively correlate with the expression of Nogo\u2010B in the intima and media (r\u00a0=\u00a0\u22120.32; p\u00a0&lt;\u00a00.05; r\u00a0=\u00a0\u22120.32; p\u00a0&lt;\u00a00.05). Macrophage infiltrates were more prominent in intima of <jats:styled-content style=\"fixed-case\">CVR<\/jats:styled-content> subjects (0.65 vs 3.52 a.u.; p\u00a0&lt;\u00a00.01). Macrophage density in intima increased with atherosclerosis progression (r\u00a0=\u00a00.37; p\u00a0&lt;\u00a00.01). CD68 macrophages density in adventitia was lower in <jats:styled-content style=\"fixed-case\">CVR<\/jats:styled-content> arteries than in healthy arteries. The expression of Nogo\u2010B, in arterial intima, is impeded in the early stages of atherosclerosis. Accumulation of arterial intimal CD68 macrophages has been shown to progress; however, the overall macrophage density in the adventitia is reduced in arteries shown to have intimal thickening. Macrophage infiltration is not accompanied by Nogo\u2010B expression in atherosclerotic arteries.<\/jats:p>","DOI":"10.1111\/apm.12212","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T05:09:53Z","timestamp":1387861793000},"page":"742-749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nogo\u2010B expression, in arterial intima, is impeded in the early stages of atherosclerosis in humans"],"prefix":"10.1111","volume":"122","author":[{"given":"Katarzyna","family":"Dro\u017cd\u017c","sequence":"first","affiliation":[{"name":"Regional Specialized Hospital in Wroclaw Research and Development Center Wroclaw Poland"},{"name":"Department of Internal Medicine 4th Military Hospital Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irmina","family":"Grzegorek","sequence":"additional","affiliation":[{"name":"Regional Specialized Hospital in Wroclaw Research 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