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Therefore, detecting dynamic expression of <jats:italic>Fhl1<\/jats:italic> and other molecules (<jats:italic>Hgf<\/jats:italic>,<jats:italic> MyoD1<\/jats:italic>,<jats:italic> Myogenin,<\/jats:italic> and <jats:italic>Myh4<\/jats:italic>) that control limb muscle development in hind limbs of different gestational age will provide a foundation for further research on the molecular mechanism involves in the myopathies or <jats:styled-content style=\"fixed-case\">CCF<\/jats:styled-content>. The dynamic gene expression levels of <jats:italic>Fhl1, Hgf<\/jats:italic>,<jats:italic> MyoD1<\/jats:italic>,<jats:italic> Myogenin,<\/jats:italic> and <jats:italic>Myh4<\/jats:italic> in the lower limbs of E16, E17, E19, and E20 rat embryos were examined by real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Immunofluorescence was used to detect formation of specific muscle fibers (fast or slow fibers) in distal E17 hind limbs. The expression levels of <jats:italic>Fhl1<\/jats:italic>,<jats:italic> Hgf<\/jats:italic>,<jats:italic> MyoD1<\/jats:italic>,<jats:italic> Myogenin,<\/jats:italic> and <jats:italic>Myh4<\/jats:italic> were varying in hind limbs of different gestational age. Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> results showed that all the genes that control skeletal muscle development except for <jats:italic>Fhl1<\/jats:italic> exhibited a peak in E17 lower limbs. Immunofluorescence results showed obviously positive fast\u2010myosin in the distal E17 lower limbs and meanwhile slow\u2010myosin had no apparently signals. E17 was a critical time point for terminal skeletal muscle differentiation in the lower limbs of rat embryos.<\/jats:p>","DOI":"10.1111\/apm.12217","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T07:41:01Z","timestamp":1391067661000},"page":"766-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dynamic expression of molecules that control limb muscle development including <i>Fhl1<\/i> in hind limbs of different gestational age"],"prefix":"10.1111","volume":"122","author":[{"given":"Wang","family":"Li\u2010Li","sequence":"first","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhao\u2010Hong","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Lian\u2010Yong","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Di","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhang","family":"Yi","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Jia\u2010Ning","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bai","family":"Yu\u2010Zuo","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Zheng\u2010Wei","sequence":"additional","affiliation":[{"name":"Key laboratory of health ministry for congenital malformation Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Wei\u2010Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Shengjing Hospital China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun","family":"Kai\u2010Lai","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-8-62"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2010.01.021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bpb.0000228385.09313.37"},{"key":"e_1_2_8_5_1","first-page":"644","article-title":"Accessory soleus muscle as a cause of congenital talipes equino varus. 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