{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T22:23:48Z","timestamp":1769552628142,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T00:00:00Z","timestamp":1391126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003035","name":"Aase and Ejnar Danielsen Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003035","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Civil engineer Lars Andersen Legat"},{"DOI":"10.13039\/501100005929","name":"Danish Eye Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005929","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fight for Sight - Denmark"},{"name":"Synoptik Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Aquaporins (<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>) are channels in the cell membrane that mainly facilitate a passive transport of water. In the eye, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s are expressed in the ciliary body and retina and may contribute to the pathogenesis of glaucoma and optic neuropathy. We investigated the expression of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>5, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 in human glaucoma eyes compared with normal eyes. Nine glaucoma eyes were examined. Of these, three eyes were diagnosed with primary open angle glaucoma; three eyes had neovascular glaucoma; and three eyes had chronic angle\u2010closure glaucoma. Six eyes with normal intraocular pressure and without glaucoma were used as control. Immunohistochemistry was performed using antibodies against <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>5, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9. For each specimen, optical densities of immunoprecipitates were measured using Photoshop and the staining intensities were calculated. Immunostaining showed labelling of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 in the nonpigmented ciliary epithelium and the staining intensities were significantly decreased in glaucoma eyes (p\u00a0=\u00a00.003; p\u00a0=\u00a00.018). <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 expression in the M\u00fcller cell endfeet was increased (p\u00a0=\u00a00.046), and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 labelling of the retinal ganglion cells (<jats:styled-content style=\"fixed-case\">RGC<\/jats:styled-content>) showed decreased intensity (p\u00a0=\u00a00.037). No difference in <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>4 and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 expression was found in the optic nerve fibres. This study is the first investigating <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s in human glaucoma eyes. We found a reduced expression of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 in the retinal ganglion cells of glaucoma eyes. Glaucoma also induced increased <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 expression in the M\u00fcller cell endfeet. In the ciliary body of glaucoma eyes, the expression of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 was reduced. Therefore, the expression of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s seems to play a role in glaucoma.<\/jats:p>","DOI":"10.1111\/apm.12225","type":"journal-article","created":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T06:59:22Z","timestamp":1391151562000},"page":"772-780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Altered aquaporin expression in glaucoma eyes"],"prefix":"10.1111","volume":"122","author":[{"given":"Thuy Linh","family":"Tran","sequence":"first","affiliation":[{"name":"Eye Pathology Institute Department of Neuroscience and Pharmacology University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toke","family":"Bek","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"la Cour","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Glostrup Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f8ren","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicin Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan Ulrik","family":"Prause","sequence":"additional","affiliation":[{"name":"Eye Pathology Institute Department of Neuroscience and Pharmacology University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Hamann","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Glostrup Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Heegaard","sequence":"additional","affiliation":[{"name":"Eye Pathology Institute Department of Neuroscience and Pharmacology University of Copenhagen Copenhagen Denmark"},{"name":"Department of Ophthalmology Glostrup Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-04332-8.00183-9"},{"key":"e_1_2_6_3_1","first-page":"3028","article-title":"Glaukompatienter i behandling hos praktiserende ojenlaeger i Danmark. 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