{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:02:50Z","timestamp":1740135770302,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T00:00:00Z","timestamp":1396569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["30973470, 81172334"],"award-info":[{"award-number":["30973470, 81172334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science & Technology Support Project (the 11th Five-Year Plan) of China","award":["2006BAI02A14"],"award-info":[{"award-number":["2006BAI02A14"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Primary gastric B\u2010cell non\u2010Hodgkin's lymphoma (B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content>) has a similar morphocytological presentation to severe chronic gastritis, which complicates the pathological diagnosis of this disease. To investigate the practicality and utility of the <jats:styled-content style=\"fixed-case\">BIOMED<\/jats:styled-content>\u20102 standardized primer system for the diagnosis of primary gastric B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content> from endoscopic biopsy specimens, we selected 65 cases of archived paraffin\u2010embedded primary gastric B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content> specimens as well as 27 cases of severe chronic gastritis samples to serve as a negative control group. The positivity rates of immunoglobulin heavy chain (IgH) gene rearrangements detected by the <jats:styled-content style=\"fixed-case\">BIOMED<\/jats:styled-content>\u20102 standardized primer system for the two groups were 86.4% and 12.0%, respectively, which are significantly different (p<jats:italic>\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Importantly, the combined detection of the five groups of the IgH primer system increased the detection rate of B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content>. These findings indicate that the <jats:styled-content style=\"fixed-case\">BIOMED<\/jats:styled-content>\u20102 standardized primer system is suitable for formalin\u2010fixed and paraffin\u2010embedded (FFPE) specimens and is valuable as a secondary diagnostic tool for primary gastric B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content> as well as the differential diagnosis of severe chronic gastritis.<\/jats:p>","DOI":"10.1111\/apm.12250","type":"journal-article","created":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T07:29:03Z","timestamp":1396596543000},"page":"873-879","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of the <scp>BIOMED<\/scp>\u20102 standardized primer system for the diagnosis of primary gastric B\u2010cell non\u2010Hodgkin's lymphoma"],"prefix":"10.1111","volume":"122","author":[{"given":"Cong\u2010Wei","family":"Jia","sequence":"first","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Mei","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 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