{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T20:17:56Z","timestamp":1778617076586,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T00:00:00Z","timestamp":1396483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Competitive Research Funding of the Tampere University Hospital"},{"name":"METOXIA"},{"name":"Sigrid Juselius Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Purpose of the study was to evaluate the presence of hypoxia\u2010inducible, tumour\u2010associated carbonic anhydrases <jats:styled-content style=\"fixed-case\">IX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">XII<\/jats:styled-content> in normal skin and a series of cutaneous tumours. Human tumour samples were taken during surgical operations performed on 245 patients and were immunohistochemically stained. A histological score value was calculated for statistical analyses which were performed using <jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content> for Windows, versions 17.0 and 20.0. In normal skin, the highest expression of <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> was detected in hair follicles, sebaceous glands, and basal parts of epidermis. <jats:styled-content style=\"fixed-case\">CA XII<\/jats:styled-content> was detected in all epithelial components of skin. Both <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CA XII<\/jats:styled-content> expression levels were significantly different in epidermal, appendigeal, and melanocytic tumour categories. Both <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">XII<\/jats:styled-content> showed the most intense immunostaining in epidermal tumours, whereas virtually all melanocytic tumours were devoid of <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">XII<\/jats:styled-content> immunostaining. In premalignant lesions, <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> expression significantly increased when the tumours progressed to more severe dysplasia forms. Both <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">XII<\/jats:styled-content> are highly expressed in different epithelial components of skin. They are also highly expressed in epidermal tumours, in which <jats:styled-content style=\"fixed-case\">CA IX<\/jats:styled-content> expression levels also correlate with the dysplasia grade. Interestingly, both isozymes are absent in melanocytic tumours.<\/jats:p>","DOI":"10.1111\/apm.12251","type":"journal-article","created":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T06:33:16Z","timestamp":1396506796000},"page":"880-889","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Expression of cancer\u2010related carbonic anhydrases <scp>IX<\/scp> and <scp>XII<\/scp> in normal skin and skin neoplasms"],"prefix":"10.1111","volume":"122","author":[{"given":"Leo","family":"Syrj\u00e4nen","sequence":"first","affiliation":[{"name":"Institute of Biomedical Technology and School of Medicine University of Tampere Tampere Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiina","family":"Luukkaala","sequence":"additional","affiliation":[{"name":"Pirkanmaa Hospital District and School of Health Sciences Science Center University of Tampere Tampere Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Leppilampi","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Oulu University Hospital Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Kallioinen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oulu and Oulu University Hospital Medical Research Center Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Pastorekova","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Virology Slovak Academy of Sciences Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaromir","family":"Pastorek","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Virology Slovak Academy of Sciences Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul","family":"Waheed","sequence":"additional","affiliation":[{"name":"Edward A. 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