{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:09:20Z","timestamp":1762506560206},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T00:00:00Z","timestamp":1432252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical University of Sofia","award":["69\/2014"],"award-info":[{"award-number":["69\/2014"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>The effect of <jats:italic>Chlamydia trachomatis<\/jats:italic>,<jats:italic> Ureaplasma urealyticum<\/jats:italic> and <jats:italic>Mycoplasma hominis<\/jats:italic> over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying <jats:styled-content style=\"fixed-case\">mRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The registered prevalence was as follows: <jats:italic>C.\u00a0trachomatis<\/jats:italic> \u2013 13.9%, <jats:italic>U.\u00a0urealyticum<\/jats:italic> \u2013 19.2%, <jats:italic>M.\u00a0hominis<\/jats:italic> \u2013 9.9%. Co\u2010infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT\u2010PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.<\/jats:p>","DOI":"10.1111\/apm.12391","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T12:30:01Z","timestamp":1432297801000},"page":"586-588","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Detection of <i>Chlamydia trachomatis<\/i>,<i> Ureaplasma urealyticum<\/i> and <i>Mycoplasma hominis<\/i> in infertile Bulgarian men with multiplex real\u2010time polymerase chain reaction"],"prefix":"10.1111","volume":"123","author":[{"given":"Vessela","family":"Ouzounova\u2010Raykova","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Medical University\u2010Sofia Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simeon","family":"Rangelov","sequence":"additional","affiliation":[{"name":"Department of Andrology University Hospital \u2018Tsaritsa Yoanna\u2019 Medical University\u2010Sofia Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iordanka","family":"Ouzounova","sequence":"additional","affiliation":[{"name":"Department of Andrology University Hospital \u2018Tsaritsa Yoanna\u2019 Medical University\u2010Sofia Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Mitov","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Medical University\u2010Sofia Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.11.024"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.067835"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.107.003566"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.05.018"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/19396361003792853"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2008.008334"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3855\/T2.2.116"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.013821"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1179\/1120009X12Z.00000000021"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-129"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapm.12391","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapm.12391","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.12391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T16:36:13Z","timestamp":1696350973000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.12391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,22]]},"references-count":10,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/apm.12391"],"URL":"https:\/\/doi.org\/10.1111\/apm.12391","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,22]]},"assertion":[{"value":"2015-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}