{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:17:44Z","timestamp":1790183864023,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,2]],"date-time":"2015-05-02T00:00:00Z","timestamp":1430524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>Brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans.<jats:italic>Brucella<\/jats:italic>spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. In this study, we examined the immune response and the protection elicited by using recombinant<jats:italic>Brucella<\/jats:italic>urease (<jats:styled-content style=\"fixed-case\">rU<\/jats:styled-content>rease) vaccination in<jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. The<jats:italic>urease<\/jats:italic>gene was cloned in<jats:styled-content style=\"fixed-case\">pET<\/jats:styled-content>28a and the resulting recombinant protein was employed as subunit vaccine. Recombinant protein was administered subcutaneously and intraperitoneally. Dosage reduction was observed with subcutaneous (<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>) vaccination when compared with intraperitoneal (<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>) vaccination.<jats:styled-content style=\"fixed-case\">rU<\/jats:styled-content>rease induced mixed Th1\u2013Th2 immune responses with high titers of specific IgG1 and IgG2a. In lymphocyte proliferation assay, splenocytes from<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010vaccinated mice displayed a strong recall proliferative response with high amounts of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 and<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 production. Vaccinated mice were challenged with virulent<jats:italic>Brucella melitensis<\/jats:italic>,<jats:italic>B.\u00a0abortus<\/jats:italic>and<jats:italic>B.\u00a0suis<\/jats:italic>. The<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>vaccination route exhibited a higher degree of protection than<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>vaccination (p value\u00a0\u2264\u00a00.05). Altogether, our results indicated that<jats:styled-content style=\"fixed-case\">rU<\/jats:styled-content>rease could be a useful antigen candidate for the development of subunit vaccines against brucellosis.<\/jats:p>","DOI":"10.1111\/apm.12400","type":"journal-article","created":{"date-parts":[[2015,5,2]],"date-time":"2015-05-02T12:18:57Z","timestamp":1430569137000},"page":"667-675","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against<i>Brucella abortus<\/i>and<i>Brucella melitensis<\/i>infections"],"prefix":"10.1111","volume":"123","author":[{"given":"Morteza","family":"Abkar","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics Faculty of Basic Sciences Tarbiat Modares University Tehran 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