{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:14:22Z","timestamp":1773306862834,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T00:00:00Z","timestamp":1432512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004892","name":"Agencia Espa\u00f1ola de Cooperaci\u00f3n Internacional al Desarrollo","doi-asserted-by":"crossref","award":["D\/024648\/09 and D\/030509\/10"],"award-info":[{"award-number":["D\/024648\/09 and D\/030509\/10"]}],"id":[{"id":"10.13039\/501100004892","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Generalitat de Catalunya, Departament d\u2019Universitats, Recerca i Societat de la Informaci\u00f3","award":["2014SGR26"],"award-info":[{"award-number":["2014SGR26"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"crossref","award":["CES11\/012"],"award-info":[{"award-number":["CES11\/012"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>Furazolidone\u2010resistant mutants were obtained from four clinical isolates of diarrhoeagenic <jats:italic>Escherichia coli<\/jats:italic>. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe\u2010Arg\u2010\u03b2\u2010Naphtylamyde. The presence of mutations in the nitroreductase genes <jats:italic>nfsA<\/jats:italic> and <jats:italic>nfsB<\/jats:italic> was analysed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>. The frequency of mutation ranged from &lt;9.6\u00a0\u00d7\u00a010<jats:sup>\u221210<\/jats:sup> to 9.59\u00a0\u00d7\u00a010<jats:sup>\u22127<\/jats:sup>. Neither an effect on efflux pumps inhibited by Phe\u2010Arg\u2010\u03b2\u2010Naphtylamyde nor cross\u2010resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase\u2010encoding genes: 17 in the <jats:italic>nfsA<\/jats:italic> gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the <jats:italic>nfsB<\/jats:italic> (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone\u2010resistant mutants, together with the lack of cross\u2010resistance with unrelated antimicrobial agents.<\/jats:p>","DOI":"10.1111\/apm.12401","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T20:13:57Z","timestamp":1432584837000},"page":"676-681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development and analysis of furazolidone\u2010resistant <i><scp>E<\/scp>scherichia coli<\/i> mutants"],"prefix":"10.1111","volume":"123","author":[{"given":"Sandra","family":"Mart\u00ednez\u2010Puchol","sequence":"first","affiliation":[{"name":"ISGlobal, Barcelona Ctr. Int. Health Res. 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