{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:00:27Z","timestamp":1785477627816,"version":"3.56.0"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T00:00:00Z","timestamp":1432512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371986"],"award-info":[{"award-number":["81371986"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301598"],"award-info":[{"award-number":["81301598"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81201426"],"award-info":[{"award-number":["81201426"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2014T70928"],"award-info":[{"award-number":["2014T70928"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2013M530427"],"award-info":[{"award-number":["2013M530427"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>This study aimed to observe the effects of Se deficiency on epiphyseal plates of two generation <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> rats fed with artificial total synthetic low Se diet. All F0 and F1 <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> rats were fed with synthetic low Se diet (SeD group) and low Se diet supplied with Se (SeS group). The levels of selenium and enzyme activities of <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x were detected in plasma of the rats. General growth of bone and articular cartilage was measured macroscopically and microscopically. The epiphyseal plate of femur heads or tibia were obtained to histological and immunohistochemical examinations. The cartilage from left knee joints and femur heads was used to detect the gene expression of collagens, <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>s and several selenoproteins by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Two generation SeD rats showed Se insufficiency status. The thicknesses of the femur and tibial epiphyseal plates in both F0 and F1 SeD rats were significantly less than that of SeS rats. In F1 generation, SeD rats showed much fewer proliferative chondrocyte layers than SeS ones. Importantly, two generation SeD rats both showed significantly more serious pathological changes of epiphyseal plates. In two generation rats, gene expressions of <jats:styled-content style=\"fixed-case\">COL II<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GP<\/jats:styled-content>x1 and <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x4 were significantly down\u2010regulated in SeD rats than SeS ones; meanwhile <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>\u20104 showed an up\u2010regulated expression in cartilage. Dietary Se deficiency can apparently cause epiphyseal plate lesion and decrease cartilage type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> collagen production and <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x1 activity in two generation <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> rats fed with the artificial total synthesis low Se diet.<\/jats:p>","DOI":"10.1111\/apm.12404","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T20:18:38Z","timestamp":1432585118000},"page":"697-705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Abnormality of epiphyseal plate induced by selenium deficiency diet in two generation <scp>DA<\/scp> rats"],"prefix":"10.1111","volume":"123","author":[{"given":"Zixin","family":"Min","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of 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