{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:46:34Z","timestamp":1790304394089,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,6,8]],"date-time":"2015-06-08T00:00:00Z","timestamp":1433721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"publisher","award":["R-52-A2569-11-S7"],"award-info":[{"award-number":["R-52-A2569-11-S7"]}],"id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>The interleukin\u20106 (IL\u20106)\/Janus kinase (JAK)\/signal transducer and activator of transcription 3 (<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3) pathway is of crucial importance in promoting tumorigenesis in several malignant tumors but may also be active in benign tumors, e.g., of pleomorphic adenoma (<jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>). In this study we characterize the expression of the pathway components with immunohistochemistry and selected <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s and micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) regulated by this pathway in isolated duct\u2010 and myoepithelial cells in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>. 46 <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>s were immunostained and 10 of these were used for <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content>). Six frozen specimens were analyzed using reverse transcription\u2010polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Using immunohistochemistry, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 were detected significantly more frequently in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content> cells than in cells from normal salivary gland tissue. Using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> cyclin D1, fibroblast growth factor 2, and p21 were found to be overexpressed while matrix metallopeptidase 9 was detected at low levels in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content> compared to normal salivary gland. <jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content> showed significant overexpression of miR\u2010181b in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>, while miR\u201021 was undetectable in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content> and normal tissue. Overexpression of the pathway components and its <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> products provide important clues regarding the growth of <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>s. Our findings brings us one step closer to targeted treatment of this tumor entity, although <jats:italic>in\u00a0vitro<\/jats:italic> studies are warranted to confirm this.<\/jats:p>","DOI":"10.1111\/apm.12407","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T02:15:20Z","timestamp":1433988920000},"page":"706-715","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Activation of the interleukin\u20106\/Janus kinase\/<scp>STAT<\/scp>3 pathway in pleomorphic adenoma of the parotid gland"],"prefix":"10.1111","volume":"123","author":[{"given":"Simon","family":"Andreasen","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head &amp; Neck Surgery and Audiology Rigshospitalet Copenhagen Denmark"},{"name":"Department of Otorhinolaryngology and Maxillofacial Surgery K\u00f8ge University Hospital K\u00f8ge Denmark"},{"name":"University of Copenhagen Copenhagen 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