{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:39Z","timestamp":1790665539670,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"\u00d6rebro County Council Research Committee and the Foundation for Medical Research at \u00d6rebro University Hospital, Sweden."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>In this study, we performed an evaluation of the new <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010marked multiplex real\u2010time AmpliSens N.gonorrhoeae\/C.trachomatis\/M.genitalium\/T.vaginalis\u2010<jats:styled-content style=\"fixed-case\">MULTIPRIME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FRT PCR<\/jats:styled-content> assay compared to <jats:styled-content style=\"fixed-case\">APTIMA<\/jats:styled-content> tests, i.e., <jats:styled-content style=\"fixed-case\">APTIMA COMBO<\/jats:styled-content> 2\u00a0assay, <jats:styled-content style=\"fixed-case\">APTIMA <\/jats:styled-content><jats:italic>Trichomonas\u00a0vaginalis<\/jats:italic> assay (<jats:styled-content style=\"fixed-case\">FDA<\/jats:styled-content>\u2010approved), and two different <jats:styled-content style=\"fixed-case\">APTIMA <\/jats:styled-content><jats:italic>Mycoplasma\u00a0genitalium<\/jats:italic> assays (research use only; one of them only used for discrepancy analysis). Vaginal swabs (n\u00a0=\u00a0209) and first\u2010void urine (<jats:styled-content style=\"fixed-case\">FVU<\/jats:styled-content>) specimens from females (n\u00a0=\u00a0498) and males (n\u00a0=\u00a0554), consecutive attendees (n\u00a0=\u00a01261) at a dermatovenerological clinic in Sweden, were examined. The sensitivity of the AmpliSens <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay for detection of <jats:italic>C.\u00a0trachomatis<\/jats:italic> (6.3% prevalence), <jats:italic>M.\u00a0genitalium<\/jats:italic> (5.7% prevalence), <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> (0.3% prevalence), and <jats:italic>T.\u00a0vaginalis<\/jats:italic> (0.08% prevalence) was 97.5% (95% confidence interval (<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>): 91.2\u201399.6%), 81.9% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 70.7\u201389.7%), 100% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 40.2\u2013100%) and 100% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 16.5\u2013100%), respectively. The specificity of the AmpliSens <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay was 100% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 99.6\u2013100%) for all agents. The analytical sensitivity and specificity for <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> detection was excellent, i.e., 55 international gonococcal strains detected and 135 isolates of 13 non\u2010gonococcal Neisseria species were negative. In conclusion, the multiplex real\u2010time AmpliSens N.gonorrhoeae\/C.trachomatis\/M.genitalium\/T.vaginalis\u2010<jats:styled-content style=\"fixed-case\">MULTIPRIME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FRT PCR<\/jats:styled-content> assay demonstrated high sensitivity and excellent specificity for the detection of <jats:italic>C.\u00a0trachomatis<\/jats:italic>,<jats:italic> N.\u00a0gonorrhoeae<\/jats:italic>, and <jats:italic>T.\u00a0vaginalis<\/jats:italic>, and excellent specificity but suboptimal sensitivity for <jats:italic>M.\u00a0genitalium<\/jats:italic> detection.<\/jats:p>","DOI":"10.1111\/apm.12430","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T15:16:18Z","timestamp":1440429378000},"page":"879-886","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Evaluation of the new AmpliSens multiplex real\u2010time <scp>PCR<\/scp> assay for simultaneous detection of <i>Neisseria\u00a0gonorrhoeae, Chlamydia\u00a0trachomatis, Mycoplasma\u00a0genitalium<\/i>, and <i>Trichomonas\u00a0vaginalis<\/i>"],"prefix":"10.1111","volume":"123","author":[{"given":"Tatiana","family":"Rumyantseva","sequence":"first","affiliation":[{"name":"Department of Molecular Diagnostics Central Research Institute for Epidemiology Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Golparian","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for Pathogenic Neisseria Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian S.","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology \u00d6rebro University Hospital \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Johansson","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for Pathogenic Neisseria Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My","family":"Falk","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology \u00d6rebro University Hospital \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Fredlund","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for Pathogenic Neisseria Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alje","family":"Van Dam","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Amsterdam Health Centre Amsterdam the Netherlands"},{"name":"Department of Medical Microbiology OLVG Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Guschin","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics Central Research Institute for Epidemiology Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for Pathogenic Neisseria Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Global Incidence and Prevalence of Selected Curable Sexually Transmitted Infections \u2010 2008","author":"World Health Organization","year":"2012"},{"key":"e_1_2_6_3_1","unstructured":"Laboratory diagnosis of sexually transmitted infections including human immunodeficiency virus. 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