{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T07:27:32Z","timestamp":1770535652153,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T00:00:00Z","timestamp":1441152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Detection of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> relies increasingly on nucleic acid amplification tests (<jats:styled-content style=\"fixed-case\">NAAT<\/jats:styled-content>s). The specificity of many gonococcal <jats:styled-content style=\"fixed-case\">NAAT<\/jats:styled-content>s has been suboptimal and supplementary testing remains recommended in Europe and several additional countries. The novel dual\u2010target GeneProof\u00a0<jats:italic>Neisseria gonorrhoeae <\/jats:italic><jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> kit, targeting <jats:italic>porA<\/jats:italic> pseudogene and 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene, showed a high specificity and sensitivity when isolates of non\u2010gonococcal <jats:italic>Neisseria<\/jats:italic> and related species (n\u00a0=\u00a0144), and gonococci (n\u00a0=\u00a0104) were tested. However, rare gonococcal <jats:italic>porA<\/jats:italic> mutants were only detected in the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene target and two non\u2010gonococcal isolates showed a low\u2010level cross\u2010reactivity in the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene target. The detection limit for both targets was 1.5 copies per reaction.<\/jats:p>","DOI":"10.1111\/apm.12440","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T20:40:46Z","timestamp":1441226446000},"page":"955-958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Analytical specificity and sensitivity of the novel dual\u2010target GeneProof\u00a0<i>Neisseria gonorrhoeae <\/i><scp>PCR<\/scp> kit for detection of <i>N. gonorrhoeae<\/i>"],"prefix":"10.1111","volume":"123","author":[{"given":"Daniel","family":"Golparian","sequence":"first","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections Swedish Reference Laboratory for Pathogenic Neisseria Department of Laboratory Medicine Faculty of Medicine and Health \u00d6rebro University \u00d6rebro 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