{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T19:49:09Z","timestamp":1767988149661,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deanship of Scientific Research, King Khalid University","award":["KKKU-MED-11-103"],"award-info":[{"award-number":["KKKU-MED-11-103"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by various types of immunological abnormalities including circulating and tissue\u2010fixed autoantibodies reactive with autoantigens. The mechanism that can explain the production of these antibodies is unclear. Here we address the binding specificity of SLE autoantibodies with recombinant alpha interferon 2b (hrIFN \u03b1\u20102b), commercially available interferon (IFN \u03b1\u20102b), and the gene (cIFN \u03b1\u20102b) encoding this interferon. hrIFN \u03b1\u20102b showed higher binding with naturally occurring SLE autoantibodies as compared to IFN \u03b1\u20102b (p\u00a0&lt;\u00a00.05) or cIFN \u03b1\u20102b gene (p\u00a0&lt;\u00a00.001) as assessed by direct binding, inhibition ELISA, and quantitative precipitin titration. The relative affinity of SLE autoantibodies for hrIFN \u03b1\u20102b, IFN \u03b1\u20102b, and cIFN \u03b1\u20102b gene was in the order of 1.13\u00a0\u00d7\u00a010<jats:sup>\u22127<\/jats:sup>, 1.38\u00a0\u00d7\u00a010<jats:sup>\u22126<\/jats:sup>, and 1.22\u00a0\u00d7\u00a010<jats:sup>\u22126<\/jats:sup>, respectively. hrIFN \u03b1\u20102b is shown to have unique epitopes that would explain the possible antigenic role of hrIFN \u03b1\u20102b in the generation of SLE autoantibodies. Anti\u2010hrIFN \u03b1\u20102b antibodies have been shown to represent an alternative immunological probe for the estimation of interferon alpha 2b in the serum of SLE patients.<\/jats:p>","DOI":"10.1111\/apm.12464","type":"journal-article","created":{"date-parts":[[2015,11,8]],"date-time":"2015-11-08T19:13:26Z","timestamp":1447010006000},"page":"1016-1024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Increased binding of circulating systemic lupus erythematosus autoantibodies to recombinant interferon alpha 2b"],"prefix":"10.1111","volume":"123","author":[{"given":"Wahid Ali","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry College of Medicine King Khalid University Abha Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javed Anwer","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry 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