{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:27:26Z","timestamp":1789864046657,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008052","name":"Fundaci\u00f3n Alfonso Mart\u00edn Escudero","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Information on the prevalence of biofilm\u2010related factors (PIA, Bhp, Aap, Embp) in <jats:italic>Staphylococcus epidermidis<\/jats:italic> of animal origin is scarce. In this study, 263 <jats:italic>S.\u00a0epidermidis<\/jats:italic> isolates of diverse origin (animal, farmers, patients, and laboratory staff) were investigated for the presence of the <jats:italic>ica<\/jats:italic> operon (<jats:italic>icaRADBC<\/jats:italic>). The <jats:italic>icaRADBC<\/jats:italic>\u2010positive isolates were further characterized by means of biofilm formation, presence of other biofilm\u2010related genes, antimicrobial resistance, and population structure. Of all isolates, 28.5% (n\u00a0=\u00a075) were <jats:italic>icaRADBC<\/jats:italic>\u2010positive, including 16.5% of animal origin, 29.1% farmer isolates, and 44.6% hospital\u2010associated isolates (including patients and laboratory staff isolates). Most <jats:italic>icaRADBC<\/jats:italic>\u2010positive isolates carried <jats:italic>embp<\/jats:italic> (n\u00a0=\u00a073), <jats:italic>aap<\/jats:italic> (n\u00a0=\u00a057), <jats:italic>bhp<\/jats:italic> (n\u00a0=\u00a022), and IS<jats:italic>256<\/jats:italic> (n\u00a0=\u00a029). Statistical differences were found between animal and patient isolates for the presence of <jats:italic>icaRADBC<\/jats:italic>,<jats:italic> bhp<\/jats:italic>, and <jats:italic>aap<\/jats:italic>. No statistically significant relation was found between the presence of one or more genes and the level of biofilm formation. Most <jats:italic>icaRADBC<\/jats:italic>\u2010positive isolates belonged to the clonal complex 5 (formerly 2) and most sequence types corresponded to types previously observed in community and nosocomial <jats:italic>S.\u00a0epidermidis<\/jats:italic> populations. Although the prevalence of <jats:italic>S.\u00a0epidermidis<\/jats:italic> in the nasal cavity of bovines and poultry is low, some isolates belong to STs related to <jats:italic>ica<\/jats:italic>\u2010positive clinical strains.<\/jats:p>","DOI":"10.1111\/apm.12472","type":"journal-article","created":{"date-parts":[[2015,11,8]],"date-time":"2015-11-08T19:13:26Z","timestamp":1447010006000},"page":"1081-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Biofilm formation of <i>ica<\/i> operon\u2010positive <i>Staphylococcus epidermidis<\/i> from different sources"],"prefix":"10.1111","volume":"123","author":[{"given":"Maria","family":"Angeles Argud\u00edn","sequence":"first","affiliation":[{"name":"Department of Bacterial Diseases Veterinary and Agrochemical Research Centre Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wannes","family":"Vanderhaeghen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Reproduction and Herd Health Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stien","family":"Vandendriessche","sequence":"additional","affiliation":[{"name":"Department of Microbiology National Reference Centre \u2013 <i>Staphylococcus aureus<\/i> H\u00f4pital Erasme Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Vandecandelaere","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Microbiology Department of Pharmaceutical analysis Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[{"name":"Department of Microbiology National Reference Centre \u2013 <i>Staphylococcus aureus<\/i> H\u00f4pital Erasme Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Coenye","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Microbiology Department of Pharmaceutical analysis Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Butaye","sequence":"additional","affiliation":[{"name":"Department of Pathology, Bacteriology, and Avian Diseases Ghent University Merelbeke Belgium"},{"name":"Department of Biomedical Sciences School of Veterinary Medicine Ross University St Kitts and Nevis West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2182"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-7775"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.01.008"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.04.019"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.56"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.3.890-896.1997"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1556\/EuJMI.3.2013.2.4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2005.04.017"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1210-1215.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.01.020"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks403"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt047"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038401"},{"key":"e_1_2_6_15_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty\u2010Second Informational Supplement","author":"Clinical and Laboratory Standards Institute","year":"2014"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30445"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6805-6810.2002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46799-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00261-08"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1128\/jcm.22.6.996-1006.1985","article-title":"Adherence of coagulase\u2010negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical","volume":"22","author":"Christensen GD","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05534-11"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2012.02877.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2005.02.003"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005855318665"},{"key":"e_1_2_6_25_1","first-page":"275","article-title":"Potential airborne microbial hazards for workers on dairy and beef cattle farms in Egypt","volume":"45","author":"Abd\u2010Elall AM","year":"2009","journal-title":"Vet Ital"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2012-5795"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5614-5619.2004"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03098-13"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01767-06"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4751-4757.2005"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg257"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.10.005"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00724-08"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.007567-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03378-12"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2012.11.001"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-13-20"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapm.12472","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.12472","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T22:13:32Z","timestamp":1696457612000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.12472"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,6]]},"references-count":36,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/apm.12472"],"URL":"https:\/\/doi.org\/10.1111\/apm.12472","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,6]]},"assertion":[{"value":"2015-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}