{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T07:16:47Z","timestamp":1695799007493},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p>The diagnosis of pleural tuberculosis continues to be a challenge due to the low sensitivity of traditional diagnostic methods. Better and more rapid tests are needed for diagnosis of pleural <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>. In this study, pleural fluids were tested with rapid test to determine <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> antigen<\/jats:italic>). Affinity chromatography was used to purify specific polyclonal antibodies against <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> antigen. Pleural samples after decontamination were treated with proteinase K. Rapid test for pleural fluids was prepared by specific antibody. Rapid test was performed on 85 pleural fluid patients. The patients had a mean age of 46.55 \u00b1 15.96 years and 38 were men. The performance of rapid test, using proteinase K, was found to be the most impressive: sensitivity 93%, specificity 94%, <jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content> 90%, and <jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content> 96% compared with adenosine deaminase test (<jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, smear, and culture. The present study did demonstrate that modified <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> rapid test can substantially improve the diagnosis of extrapulmonary <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/apm.12490","type":"journal-article","created":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T08:39:13Z","timestamp":1450859953000},"page":"201-207","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Modified <scp>TB<\/scp> rapid test by proteinase K for rapid diagnosis of pleural tuberculosis"],"prefix":"10.1111","volume":"124","author":[{"given":"Shamsi","family":"Yari","sequence":"first","affiliation":[{"name":"Tuberculosis Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Hadizadeh Tasbiti","sequence":"additional","affiliation":[{"name":"Tuberculosis Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa","family":"Ghanei","sequence":"additional","affiliation":[{"name":"Tuberculosis Department Pasteur Institute of Iran Tehran Iran"},{"name":"Chemical Injury Research Center Baqiyatallah University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Ali","family":"Shokrgozar","sequence":"additional","affiliation":[{"name":"National Cell Bank of Iran Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abolfazl","family":"Fateh","sequence":"additional","affiliation":[{"name":"Tuberculosis Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Yari","sequence":"additional","affiliation":[{"name":"Blood transfusion research center High Institute for research and education in transfusion medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmadreza","family":"Bahrmand","sequence":"additional","affiliation":[{"name":"Tuberculosis Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Health Organization.Tuberculosis. 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