{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T20:23:41Z","timestamp":1778963021140,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T00:00:00Z","timestamp":1454544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>Coagulase negative staphylococci (Co<jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>) are nosocomial pathogens that cause indwelling medical device associated infections due to its biofilm forming potential and multiple antibiotic resistance. The current study focused on species identification, antibiotic resistance profile and molecular basis of biofilm formation and attachment of Co<jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content> isolated from clinical samples. Along with this, molecular screening for <jats:italic>mec<\/jats:italic>A and newly identified surface colonization protein encoded by <jats:italic>sas<\/jats:italic>X gene was also conducted. <jats:italic>S. epidermidis<\/jats:italic> (n = 19, 47%) was identified as the most prevalent Co<jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content> species and very interestingly two biofilm forming, <jats:italic>mec<\/jats:italic>A positive <jats:italic>S. epidermidis<\/jats:italic> isolates were found to carry all the biofilm associated genes screened in this study, which indicates its potential to form the strong biofilm. Another novel observation of the study is the detection of <jats:italic>sas<\/jats:italic>X gene in one biofilm positive <jats:italic>S. epidermidis<\/jats:italic> isolate. The study also identified one doxycycline resistant <jats:italic>mec<\/jats:italic>A positive, multidrug resistant <jats:italic>S. haemolyticus<\/jats:italic> isolate. In conclusion, the study signifies the existence of multiple biofilm related genes, multidrug resistance and the presence of <jats:italic>sas<\/jats:italic>X gene among clinical isolates of Co<jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/apm.12510","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T04:20:12Z","timestamp":1454646012000},"page":"319-326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Studies on prevalence of biofilm associated genes and primary observation on <i>sas<\/i>X gene in clinical isolates of coagulase negative staphylococci (CoNS)"],"prefix":"10.1111","volume":"124","author":[{"given":"Kandammuriyil Radhakrishnan","family":"Soumya","sequence":"first","affiliation":[{"name":"School of Biosciences Mahatma Gandhi University Kottayam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiji","family":"Mathew","sequence":"additional","affiliation":[{"name":"School of Biosciences Mahatma Gandhi University Kottayam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheela","family":"Sugathan","sequence":"additional","affiliation":[{"name":"MOSC Medical College Kolencherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyothis","family":"Mathew","sequence":"additional","affiliation":[{"name":"School of Biosciences Mahatma Gandhi University Kottayam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edayileveettil Krishnankutty","family":"Radhakrishnan","sequence":"additional","affiliation":[{"name":"School of Biosciences Mahatma Gandhi University Kottayam India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.108712"},{"key":"e_1_2_6_3_1","first-page":"448","article-title":"Coagulase negative Staphylococci and their participation in pathogenesis of human infections","volume":"107","author":"Longauerova A","year":"2006","journal-title":"Bratisl Lek Listy"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-1031-7_2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/378492"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3348-3350.2003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04515.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.9.2888-2896.2001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06981.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.3081-3083.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4101774.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2003.00544.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2692"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200112"},{"key":"e_1_2_6_15_1","first-page":"389","article-title":"Antibiotic resistance genes and susceptibility pattern in Staphylococci","volume":"135","author":"Duran N","year":"2012","journal-title":"Indian J Med Res"},{"key":"e_1_2_6_16_1","first-page":"223","article-title":"Methicillin\u2010resistant Staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial development","volume":"83","author":"Morell EA","year":"2010","journal-title":"Yale Journal of Biology and Medicine"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/427701"},{"key":"e_1_2_6_18_1","first-page":"541","article-title":"Genotypic by icaA gene based PCR of Staphylococcal strains isolated from bovine mastitis cases","volume":"12","author":"Kuler RN","year":"2013","journal-title":"Indian J Biotechnol"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00488-11"},{"key":"e_1_2_6_20_1","first-page":"13","article-title":"Methicillin \u2013resistant Staphylococcus epidermidis carrying biofilm formation genes: detection of clinical isolates by multiplex PCR","volume":"14","author":"Iorio NL","year":"2011","journal-title":"Int Microbiol"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-240"},{"key":"e_1_2_6_22_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; 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