{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:47:50Z","timestamp":1777430870348,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T00:00:00Z","timestamp":1461283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009752","name":"Futurum","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009752","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Academy of Healthcare"},{"name":"Region J\u00f6nk\u00f6ping County"},{"name":"Da Nang University of Medical Technology and Pharmacy"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>We surveyed the prevalence of <jats:italic>Dientamoeba fragilis<\/jats:italic>,<jats:italic> Giardia duodenalis, Entamoeba histolytica, Entamoeba dispar<\/jats:italic>, and <jats:italic>Cryptosporidium spp<\/jats:italic> in individuals with and without gastrointestinal (<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>) symptoms residing in and around Da Nang city, Vietnam. Fecal samples were collected from children (<jats:italic>n<\/jats:italic> = 100) and adults (<jats:italic>n<\/jats:italic> = 80) with <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> symptoms and from healthy individuals (<jats:italic>n<\/jats:italic> = 88) reporting no <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> symptoms. Parasite detection was performed by multiplex real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Overall, except for <jats:italic>G. duodenalis<\/jats:italic>, we found a low prevalence (&lt;5%) of <jats:italic>D. fragilis<\/jats:italic> and <jats:italic>E. dispar<\/jats:italic> and no detection of <jats:italic>E. histolytica<\/jats:italic> and <jats:italic>C. spp<\/jats:italic> in all participants with <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> symptoms. Specifically for <jats:italic>D. fragilis<\/jats:italic> this contrasts with findings in European populations of children with <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> symptoms showing prevalence up to 73%. Moreover, our results indicate that the prevalence of <jats:italic>G. duodenalis<\/jats:italic> is higher in patients with <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> symptoms compared to asymptomatic individuals and this difference is most obvious in young patients.<\/jats:p>","DOI":"10.1111\/apm.12535","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T20:23:16Z","timestamp":1461356596000},"page":"529-533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevalence of <i>Dientamoeba fragilis, Giardia duodenalis, Entamoeba histolytica\/dispar<\/i>, and <i>Cryptosporidium spp<\/i> in Da Nang, Vietnam, detected by a multiplex real\u2010time PCR"],"prefix":"10.1111","volume":"124","author":[{"given":"Jessica","family":"\u00d6gren","sequence":"first","affiliation":[{"name":"Division of Medical Diagnostics Clinical Microbiology Laboratory County Hospital Ryhov 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