{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T20:00:45Z","timestamp":1771531245458,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T00:00:00Z","timestamp":1461283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401236"],"award-info":[{"award-number":["81401236"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is an opportunistic human pathogen that can cause severe infections in immunocompromised individuals. Because it forms biofilms, which protect against host immune attack and increase resistance to conventional antibiotics, mucoid <jats:italic>P. aeruginosa<\/jats:italic> is nearly impossible to eradicate. Moreover, mucoid conversion of <jats:italic>P. aeruginosa<\/jats:italic> in cystic fibrosis (<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>) patients leads to poor outcomes. This conversion is mainly due to <jats:italic>mucA<\/jats:italic> gene mutation, which is thought to be induced by polymorphonuclear leukocytes (<jats:styled-content style=\"fixed-case\">PMN<\/jats:styled-content>s) and the reactive oxygen species they release. Ambroxol, a mucolytic agent with antioxidant characteristics, is used clinically, and this compound has recently been demonstrated to possess anti\u2010biofilm properties. In this study, we found that ambroxol inhibits the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010mediated conversion of <jats:italic>P. aeruginosa<\/jats:italic> from a non\u2010mucoid to a mucoid phenotype, an effect that is due to its antioxidant property against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Furthermore, the bactericidal activity of ciprofloxacin against mucoid <jats:italic>P. aeruginosa<\/jats:italic> biofilms was increased <jats:italic>in vitro<\/jats:italic> when used in combination with ambroxol.<\/jats:p>","DOI":"10.1111\/apm.12542","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T21:53:12Z","timestamp":1461361992000},"page":"611-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ambroxol inhibits mucoid conversion of <i>Pseudomonas aeruginosa<\/i> and contributes to the bactericidal activity of ciprofloxacin against mucoid <i>P. aeruginosa<\/i> biofilms"],"prefix":"10.1111","volume":"124","author":[{"given":"Wenlei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of PICU Children's Hospital of Chongqing Medical University Ministry of Education Key Laboratory of Child Development and Disorders Key Laboratory of Pediatrics in Chongqing 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