{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:44:44Z","timestamp":1791423884744,"version":"4.3.3"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T00:00:00Z","timestamp":1464652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Lactobacillus crispatus<\/jats:italic> is one of the most predominant species in the healthy vagina microbiota. Nevertheless, the interactions between this commensal bacterium and the immune system are largely unknown. Given the importance of the dendritic cells (DC<\/jats:styled-content>s) in the regulation of the immunity, this study was performed to elucidate the influence of vaginal isolated L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content> from healthy Iranian women on DC<\/jats:styled-content>s, either directly by exposure of DC<\/jats:styled-content>s to ultraviolet\u2010inactivated (UVI<\/jats:styled-content>) and heat\u2010killed (HK<\/jats:styled-content>) L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content> or indirectly to its cell\u2010free supernatant (CFS<\/jats:styled-content>), and the outcomes of immune response. In this work we showed that L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content> induced strong dose\u2010dependent activation of dendritic cells and production of high levels of IL<\/jats:styled-content>\u201010, whereas IL<\/jats:styled-content>\u201012p70 production was induced at low level in an inverse dose\u2010dependent manner. This stimulation skewed T cells polarization toward CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>25+<\/jats:sup> FOXP<\/jats:styled-content>3+<\/jats:sup> Treg cells and production of IL<\/jats:styled-content>\u201010 in a dose\u2010dependent manner in mixed leukocyte reaction (MLR<\/jats:styled-content>) test. The mode of bacterial inactivation did not affect the DC<\/jats:styled-content>s activation pattern, upon encounter with L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content>. Moreover, while DC<\/jats:styled-content>s stimulated with CFS<\/jats:styled-content> showed moderate phenotypic maturation and IL<\/jats:styled-content>\u201010 production, it failed to skew T cells polarization toward CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>25+<\/jats:sup> FOXP<\/jats:styled-content>3+<\/jats:sup> regulatory T cells (Treg) and production of IL<\/jats:styled-content>\u201010. This study showed that L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content> confers an anti\u2010inflammatory phenotype to DC<\/jats:styled-content>s through up\u2010regulation of anti\u2010inflammatory\/regulatory IL<\/jats:styled-content>\u201010 cytokine production and induction of CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>25+<\/jats:sup> FOXP<\/jats:styled-content>3+<\/jats:sup> T cells at optimal dosage. Our findings suggest that L. crispatus <\/jats:italic>SJ<\/jats:styled-content>\u20103C\u2010US<\/jats:styled-content> could be a potent candidate as protective probiotic against human immune\u2010mediated pathologies, such as chronic inflammation, vaginitis or pelvic inflammatory disease (PID<\/jats:styled-content>).<\/jats:p>","DOI":"10.1111\/apm.12556","type":"journal-article","created":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T04:22:47Z","timestamp":1464754967000},"page":"697-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Lactobacillus crispatus<\/i> strain SJ\u20103C\u2010US induces human dendritic cells (DCs) maturation and confers an anti\u2010inflammatory phenotype to DCs"],"prefix":"10.1111","volume":"124","author":[{"given":"Solat","family":"Eslami","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology School of Advanced Technologies in Medicine Tehran University 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