{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:47:25Z","timestamp":1789390045830,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,7,25]],"date-time":"2016-07-25T00:00:00Z","timestamp":1469404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>To determine the association of platelets with hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>) growth and its metastasis. We examined platelets, laboratory, and radiological data of consecutive 420 <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> and 1008 cirrhosis cases. Follow\u2010up information of platelet count in cirrhosis to <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>, pre\u2010 to post\u2010therapy, and post\u2010therapy to <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> outcome was analyzed. Cytokine profiling was performed in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> and cirrhosis (<jats:italic>n<\/jats:italic> = 10 each). On the basis of imaging, <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> was divided into six subgroups. Cytosmears of <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> were assessed for platelet clustering around tumor cells. An <jats:italic>in vitro<\/jats:italic> Matrigel invasion assay was performed on human <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cell lines using graded concentration of platelets. Baseline platelet numbers and platelet\/lymphocyte ratios (<jats:styled-content style=\"fixed-case\">PLR<\/jats:styled-content>s) were significantly higher (p &lt; 0.001) in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> than cirrhosis. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">FGF<\/jats:styled-content>, G\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>, thrombopoietin, and <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> were higher in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> than cirrhosis. Platelet counts were increased after <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> conversion of cirrhosis (p &lt; 0.001) and decreased (p &lt; 0.001) after therapy. Platelets and <jats:styled-content style=\"fixed-case\">PLR<\/jats:styled-content> in recurrence cases were higher than in responders at baseline. <jats:styled-content style=\"fixed-case\">AFP<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PIVKAII<\/jats:styled-content>, platelets, and <jats:styled-content style=\"fixed-case\">PLR<\/jats:styled-content> increase (p &lt; 0.001 each) with advancement in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> growth. Multivariate analysis showed platelets (p = 0.002), <jats:styled-content style=\"fixed-case\">PLR<\/jats:styled-content> (p = 0.004), and <jats:styled-content style=\"fixed-case\">AFP<\/jats:styled-content> (p &lt; 0.001) associated with distant metastasis. Platelet clustering seen in 75.7% of <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> group 3, 45% in group 2, and 12.5% in group 1 cases (p &lt; 0.001). Invaded cells in Matrigel assay positively correlated with platelet concentration. Platelets can contribute to the development, growth, invasion, and metastasis of <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>. Rising platelet count after <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> therapy is indicative of incomplete response or recurrence.<\/jats:p>","DOI":"10.1111\/apm.12574","type":"journal-article","created":{"date-parts":[[2016,7,26]],"date-time":"2016-07-26T04:16:43Z","timestamp":1469506603000},"page":"776-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Platelets contribute to growth and metastasis in hepatocellular carcinoma"],"prefix":"10.1111","volume":"124","author":[{"given":"Chhagan","family":"Bihari","sequence":"first","affiliation":[{"name":"Department of Pathology Institute of Liver and Biliary Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Liver and Biliary Sciences New Delhi 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