{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:58:27Z","timestamp":1760385507604},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T00:00:00Z","timestamp":1470614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7082016"],"award-info":[{"award-number":["7082016"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p>Bactericidal\/permeability increasing (BPI) is an antibiotic protein which kills Gram\u2010negative bacteria and neutralizes endotoxin. We have previously developed a recombinant adeno\u2010associated virus which contains human BPI amino acid residues 1\u2013199 and Fc fragment of human IgG1 gene (AAV\u2010hBPI\u2010Fc) and shown that the recombinant virus can protect mice from lethal endotoxemia. However, whether AAV\u2010hBPI\u2010Fc can be used <jats:italic>in vivo<\/jats:italic> for the long term remains unclear. To address this, we established an adeno\u2010associated virus\u2010containing mouse BPI and Fc fragment genes (muBPI\u2010Fc) and compared antigenicity of these recombinant proteins in murine models. Immunohistochemistry showed the expression of both fusion proteins at injected sites. ELISA and Western blotting showed that the muBPI\u2010Fc protein was detected in serum up to 8 weeks after injection, without generation of autoantibodies against muBPI\u2010Fc. In contrast, expressed hBPI\u2010Fc protein was only detected on the 2nd week, whereas the autoantibody against hBPI\u2010Fc protein occurred in serum from the 4th week to the end of study. muBPI\u2010Fc also reduced production of proinflammatory cytokines and protected mice from endotoxemia and bacteremia. Our data showed that AAV\u2010muBPI\u2010Fc has potential long\u2010term efficacy as an anti\u2010endotoxin and has anti\u2010bacterial activity in mice, suggesting the potential clinical application of AAV\u2010hBPI\u2010Fc, such as in endotoxin shock.<\/jats:p>","DOI":"10.1111\/apm.12582","type":"journal-article","created":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T19:06:00Z","timestamp":1470683160000},"page":"888-895","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Long\u2010term anti\u2010endotoxin\/<i>E. coli<\/i> efficacy in mice transfected with AAV2\/1\u2010muBPI<sub>25<\/sub>\u2010muFc\u03b31"],"prefix":"10.1111","volume":"124","author":[{"given":"Zhe","family":"Lv","sequence":"first","affiliation":[{"name":"The Department of Immunology and The Research Centre of Microbiome Capital Medical University Beijing 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