{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:28:18Z","timestamp":1761766098705},"reference-count":17,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005423","name":"Akademiska Sjukhuset","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>A duplex real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> based on the <jats:italic>rnpB<\/jats:italic> gene was developed for <jats:italic>Mycobacterium<\/jats:italic> spp. The assay was specific for the <jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex (<jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content>) and also detected all 19 tested species of non\u2010tuberculous mycobacteria (<jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content>). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other non\u2010respiratory body sites. <jats:italic>M. tuberculosis<\/jats:italic> was detected by culture in 40 samples and in 30 samples by the assay. The <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> assay showed a sensitivity similar to Roche Cobas Amplicor <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non\u2010tuberculous mycobacteria detected by culture. 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