{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:04:23Z","timestamp":1774494263634,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008403","name":"Suomalainen L\u00e4\u00e4k\u00e4riseura Duodecim","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008403","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Doublecortin\u2010like kinase 1 (<jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1) is a microtubule\u2010associated kinase. In murine intestine, <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1 marks tuft cells with characteristic microvilli, features of neuroendocrine cells and also quiescent stem cell\u2010like properties. The occurrence and pathological role of <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1\u2010positive cells in human intestinal mucosa is unknown. We analysed <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1 expression in healthy duodenal, jejunal and colorectal mucosa samples (n = 35), and in duodenal specimens from patients with coeliac disease (n = 20). The samples were immunohistochemically double\u2010stained with <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1, and synaptophysin, chromogranin A and Ki\u201067. Ultrastructure of <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1\u2010expressing duodenal cells was assessed using correlative light and electron microscopy. <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1 expression was seen in about 1% of epithelial cells diffusely scattered through the intestinal epithelium. Electron microscopy showed that the duodenal <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1\u2010positive cells had short apical microvilli similar to neighbouring enterocytes and cytoplasmic granules on the basal side. <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1\u2010positive cells were stained with synaptophysin. The number of <jats:styled-content style=\"fixed-case\">DCLK<\/jats:styled-content>1\u2010positive cells was decreased in villus atrophy in coeliac disease. Our findings indicate that in human intestinal epithelium, <jats:styled-content style=\"fixed-case\">DLCK<\/jats:styled-content>1\u2010positive cells form a subpopulation of non\u2010proliferating neuroendocrine cells with apical brush border similar to that in enterocytes, and their number is decreased in untreated coeliac disease.<\/jats:p>","DOI":"10.1111\/apm.12599","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T09:16:56Z","timestamp":1475054216000},"page":"958-965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Doublecortin\u2010like kinase 1\u2010positive enterocyte \u2013 a new cell type in human intestine"],"prefix":"10.1111","volume":"124","author":[{"given":"Joni","family":"Lepp\u00e4nen","sequence":"first","affiliation":[{"name":"Department of Pathology University of Oulu Oulu Finland"},{"name":"Department of Surgery University of Oulu Oulu 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