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We evaluated a recombinant subunit vaccine candidate based on the major outer membrane protein variable segments 2 and 4 (<jats:styled-content style=\"fixed-case\">MOMP VS<\/jats:styled-content>2\/4). To achieve maximal immunogenicity and ease of production and purification, MOMP VS2\/4 was constructed by using highly immunogenic sequences of MOMP only, thereby minimizing the presence of hydrophobic regions, and spacing the immunogenic epitopes with a flexible amino acid sequence. A purification tag was also added. The <jats:styled-content style=\"fixed-case\">MOMP VS<\/jats:styled-content>2\/4 was given intranasally, with or without intravaginal boost, with cholera toxin (<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) adjuvant to C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice, which were screened for immunogenicity and protection against a live challenge infection with <jats:italic>C.\u00a0trachomatis<\/jats:italic> serovar D. Bacterial shedding, cell\u2010mediated responses, and antibody responses were monitored. Immunized mice exhibited significantly less bacterial shedding and were better protected against infertility as compared to unimmunized control mice. Immunizations stimulated both systemic and local specific antibody (IgG1, IgG2c, and IgA) responses, and primed T cells that produced interferon\u2010\u03b3 and interleukins 13 and 17 upon challenge with recall antigen. Thus, <jats:styled-content style=\"fixed-case\">MOMP VS<\/jats:styled-content>2\/4, in combination with <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> adjuvant, stimulated Th1, Th2, and Th17 effector cells, and generated protective immunity associated with less pathology. We regard <jats:styled-content style=\"fixed-case\">MOMP VS<\/jats:styled-content>2\/4 as a promising candidate for further development into a mucosal chlamydial vaccine.<\/jats:p>","DOI":"10.1111\/apm.12605","type":"journal-article","created":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T05:05:27Z","timestamp":1479445527000},"page":"1078-1086","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protection against genital tract <i>Chlamydia trachomatis<\/i> infection following intranasal immunization with a novel recombinant <scp>MOMP VS<\/scp>2\/4 antigen"],"prefix":"10.1111","volume":"124","author":[{"given":"Ronza","family":"Hadad","sequence":"first","affiliation":[{"name":"\u00d6rebro Life Science Center School of Science and Technology \u00d6rebro University \u00d6rebro Sweden"},{"name":"Department of Laboratory Medicine Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Marks","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Gothenburg Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Kalbina","sequence":"additional","affiliation":[{"name":"\u00d6rebro Life Science Center School of Science and Technology \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Sch\u00f6n","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Gothenburg Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils","family":"Lycke","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Gothenburg Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c5ke","family":"Strid","sequence":"additional","affiliation":[{"name":"\u00d6rebro Life Science Center School of Science and Technology \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f6ren","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,18]]},"reference":[{"unstructured":"World Health Organization Department of Reproductive Health and Research.Prevalence and incidence of selected sexually transmitted infections. 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