{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T07:25:16Z","timestamp":1774682716653,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T00:00:00Z","timestamp":1479427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["80\/653\/2010\u2010ECD\u2010I"],"award-info":[{"award-number":["80\/653\/2010\u2010ECD\u2010I"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>To determine the prevalence and distribution of <jats:italic>Chlamydia trachomatis<\/jats:italic> genovars in patients with infertility by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> and <jats:italic>omp<\/jats:italic>A gene sequencing. Prevalence of other etiological agents (viz., <jats:italic>Ureaplasma<\/jats:italic> spp. and <jats:italic>Mycoplasma hominis<\/jats:italic>) were also assessed. Endocervical swabs were collected from 477 women and urine was collected from 151 men attending the Infertility Clinic. The samples were screened for <jats:italic>C.\u00a0trachomatis<\/jats:italic> by cryptic plasmid, <jats:italic>omp<\/jats:italic>A gene and nested <jats:italic>omp<\/jats:italic>A gene <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Genotyping was performed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> and sequencing. Samples were screened for <jats:italic>Ureaplasma<\/jats:italic> spp. and <jats:italic>M.\u00a0hominis<\/jats:italic>. The prevalence of <jats:italic>C.\u00a0trachomatis<\/jats:italic> in infertile women and their male partners were 15.7% (75 of 477) and 10.0% (15 of 151) respectively. Secondary infertility was significantly associated with chlamydial infection. Genovar E was the most prevalent followed by genovar D and F. Twenty\u2010four <jats:italic>C.\u00a0trachomatis<\/jats:italic> strains were selected for <jats:italic>omp<\/jats:italic>A gene sequencing. No mixed infection was picked. Variability in <jats:italic>omp<\/jats:italic>A sequences was seen in 50.0%. Both <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> and <jats:italic>omp<\/jats:italic>A gene sequencing showed concordant results. High prevalence of <jats:italic>C.\u00a0trachomatis<\/jats:italic> in infertile couples warrants routine screening for <jats:italic>C.\u00a0trachomatis<\/jats:italic> infection in all infertile couples. Genotyping of the <jats:italic>omp<\/jats:italic>A gene of <jats:italic>C.\u00a0trachomatis<\/jats:italic> may be a valuable tool in understanding the natural history of <jats:italic>C.\u00a0trachomatis<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/apm.12622","type":"journal-article","created":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T05:05:27Z","timestamp":1479445527000},"page":"1109-1115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevalence and distribution of <i>Chlamydia trachomatis<\/i> genovars in Indian infertile patients: a pilot study"],"prefix":"10.1111","volume":"124","author":[{"given":"Jyoti","family":"Rawre","sequence":"first","affiliation":[{"name":"Department of Microbiology AIIMS New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benu","family":"Dhawan","sequence":"additional","affiliation":[{"name":"Department of Microbiology AIIMS New Delhi 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