{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T07:28:26Z","timestamp":1720337306178},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T00:00:00Z","timestamp":1494460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Zhejiang Province Science and Technology","award":["2013C33146"],"award-info":[{"award-number":["2013C33146"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>The pandemic influenza A (H1N1)pdm09 virus continues to be a threat to human health. Low doses of mannan\u2010binding lectin (MBL) (&lt;1 \u03bcg\/mL) were shown not to protect against influenza A(H1N1)pdm09 infection. However, the effect of high doses of MBL has not been investigated. Dendritic cell\u2010specific intercellular adhesion molecule\u20103 grabbing non\u2010integrin (DC\u2010SIGN) has been proposed as an alternative receptor for influenza A(H1N1)pdm09 virus. In this study, we examined the expression of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> on <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s as well as on acute monocytic leukemia cell line, <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101. High doses of recombinant or human <jats:styled-content style=\"fixed-case\">MBL<\/jats:styled-content> inhibited binding of influenza A(H1N1)pdm09 to both these cell types in the presence of complement derived from bovine serum. Further, anti\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> monoclonal antibody inhibited binding of influenza A(H1N1)pdm09 to both <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content>\u2010expressing <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 cells. This study demonstrates that high doses of <jats:styled-content style=\"fixed-case\">MBL<\/jats:styled-content> can inhibit binding of influenza A(H1N1)pdm09 virus to <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content>\u2010expressing cells in the presence of complement. Our results suggest that <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> may be an alternative receptor for influenza A(H1N1)pdm09 virus.<\/jats:p>","DOI":"10.1111\/apm.12695","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T16:54:47Z","timestamp":1494521687000},"page":"655-664","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["High doses of recombinant mannan\u2010binding lectin inhibit the binding of influenza A(H1N1)pdm09 virus with cells expressing DC\u2010SIGN"],"prefix":"10.1111","volume":"125","author":[{"given":"Lei","family":"Yu","sequence":"first","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Laboratory of Cancer Biology Sir Runrun Shaw Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqiang","family":"Shang","sequence":"additional","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Tao","sequence":"additional","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pingxiang Maternal and Child Health Hospital Pingxiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinghu","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Infection Disease Zhejiang Key Laboratory for Neonatal Diseases Children Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,11]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization.Global Alert and Response (GAR). 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