{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:35:01Z","timestamp":1789997701754,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T00:00:00Z","timestamp":1492732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Nonalcoholic fatty liver disease (NAFLD<\/jats:styled-content>) is a spectrum of liver disorders ranging from simple hepatic steatosis up to nonalcoholic steatohepatitis (NASH<\/jats:styled-content>) evolving to cirrhosis and hepatocellular carcinoma (HCC<\/jats:styled-content>). Liver biopsy is still the gold standard modality for diagnosing and staging NAFLD<\/jats:styled-content>. The linkage between intestinal microbiota and NAFLD<\/jats:styled-content>, might suggest a potential role of serum zonulin in NAFLD<\/jats:styled-content> diagnosis. To appraise the role of circulating zonulin in NAFLD<\/jats:styled-content> pathogenesis, 56 subjects with proved NAFLD by ultrasonography and liver biopsy, as well as 20 healthy controls were tested. Liver function tests, serum glucose, fasting insulin, C peptide, lipid profile, homeostasis model assessment of insulin resistance (HOMA\u2010IR), IL\u20106, and circulating zonulin were performed to all subjects. Aspartate transaminase (AST<\/jats:styled-content>), alanine transaminase (ALT<\/jats:styled-content>), gamma\u2010glutamyl transferase (GGT<\/jats:styled-content>), triglycerides, HDL<\/jats:styled-content>\u2010c, fasting insulin, C peptide, HOMA<\/jats:styled-content>\u2010IR<\/jats:styled-content>, IL<\/jats:styled-content>\u20106, and serum zonulin were higher in NAFLD<\/jats:styled-content> group than in controls (p\u00a0<\u00a00.05), and in NASH<\/jats:styled-content> patients than those with simple steatosis (p\u00a0<\u00a00.05). Zonulin was positively correlated with body mass index (BMI<\/jats:styled-content>), ALT<\/jats:styled-content>, triglycerides, fasting insulin, HOMA<\/jats:styled-content>\u2010IR<\/jats:styled-content>, liver histopathology, and serum IL<\/jats:styled-content>\u20106 (p\u00a0<\u00a00.05), with inverse correlation to HDL<\/jats:styled-content>\u2010C (p\u00a0<\u00a00.05). At cut off 8.3\u00a0pc\/mL, serum zonulin was found to be of diagnostic value of NASH<\/jats:styled-content> occurrence with 100% sensitivity and specificity (AUR\u00a0=\u00a01.000, p\u2010value\u00a0=\u00a0<0.001). The increasing zonulin levels in NAFLD<\/jats:styled-content> patients with steep rise in NASH<\/jats:styled-content> group denotes a possible role in pathogenesis of NAFLD<\/jats:styled-content> occurrence and progression. This could open a new avenue of implicating zonulin antagonists as targeted therapies in NAFLD<\/jats:styled-content> prevention.<\/jats:p>","DOI":"10.1111\/apm.12696","type":"journal-article","created":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T13:51:29Z","timestamp":1492782689000},"page":"607-613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Evaluation of circulating zonulin as a potential marker in the pathogenesis of nonalcoholic fatty liver disease"],"prefix":"10.1111","volume":"125","author":[{"given":"Olfat M.","family":"Hendy","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology National Liver Institute Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha M.","family":"Elsabaawy","sequence":"additional","affiliation":[{"name":"Department of Hepatology National Liver Institute Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona M.","family":"Aref","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry National Liver Institute Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma M.","family":"Khalaf","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry National Liver Institute Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel Moaty A.","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Hepatology National Liver Institute Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmy M.","family":"El Shazly","sequence":"additional","affiliation":[{"name":"Department of Hepatology National Liver Institute Menoufia University Menoufia 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