{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:18:00Z","timestamp":1763389080357},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007083","name":"Orionin Tutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>This study evaluates the suitability of Xpert <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> (Cepheid, Sunnyvale, <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA<\/jats:styled-content>) test for cervical and tonsillar formalin\u2010fixed paraffin\u2010embedded (<jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content>) tissue samples as compared to the tests currently used in diagnostics. Cervical biopsies and liquid cytology (<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>) samples were collected from 48 women attending colposcopy. Biopsies were processed for histology and tested for hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> using Xpert <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> samples were tested using Xpert and Hybrid Capture 2 (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2; Qiagen, Hilden, Germany) tests. Also 29 archived tonsillar carcinoma samples were tested using Xpert, and the results were compared with histology and immunohistochemical p16<jats:styled-content style=\"fixed-case\">INK<\/jats:styled-content>4a (p16) staining. Among valid cervical <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> samples 46.8% were hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> positive using Xpert test and 55.3% with <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2. The sensitivity of Xpert was 84.6% as compared to <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2, and overall test concordance was 91.5%. Test concordance between valid Xpert results from biopsies and <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> samples was 84.6%. Among valid tonsillar samples 70.4% were hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> positive, and concordance of 96.3% was found between Xpert and p16 staining. To conclude, Xpert <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> test cartridge provides a convenient platform to test individual samples, including <jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content> samples. Further studies are needed to establish whether test sensitivity is sufficient to reliably differentiate between hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> positive and hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> negative head and neck carcinomas.<\/jats:p>","DOI":"10.1111\/apm.12727","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T06:32:44Z","timestamp":1500877964000},"page":"910-915","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Testing for high\u2010risk <scp>HPV<\/scp> in cervical and tonsillar paraffin\u2010embedded tissue using a cartridge\u2010based assay"],"prefix":"10.1111","volume":"125","author":[{"given":"Elina","family":"Virtanen","sequence":"first","affiliation":[{"name":"Department of Virology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pekka","family":"Laurila","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaana","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pekka","family":"Nieminen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eeva","family":"Auvinen","sequence":"additional","affiliation":[{"name":"Department of Virology University of Helsinki and Helsinki University Hospital Helsinki 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