{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:20:26Z","timestamp":1788272426193,"version":"build-2803163510"},"reference-count":79,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T00:00:00Z","timestamp":1511136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Brucellosis caused by the bacterium <jats:italic>Brucella<\/jats:italic> affects various domestic and wild species. The outer membrane proteins 25 and 31 play key roles on stimulation of cell\u2010mediated immune response against <jats:italic>Brucella<\/jats:italic>. Gro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> as one of the major <jats:italic>Brucella<\/jats:italic> antigens stimulates the immune system and increases intracellular survival of bacteria. In the present study, we assumed injection of Gro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> in combination with <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>25 and <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>31 would offer higher immunity levels. So, the impact of Gro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> with different concentrations of recombinant outer membrane proteins emulsified in Chitosan Nanoparticles on immune responses was evaluated in mice model. Results showed both univalent (except <jats:styled-content style=\"fixed-case\">rG<\/jats:styled-content>ro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content>) and divalent immunized groups induced higher <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 titers in comparison to negative control groups. While Gro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> showed negative effect on <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 titer, there were positive increase trends in <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 in some treatments. Analysis of humoral antibody response revealed both univalent and divalent immunized groups induced higher IgG2a titer than IgG1 titer, indicating strong bent of Th1 immune response. Also, results showed Gro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> can have positive impact on lymphocyte proliferation response. Overall, mice immunization using individual <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>25 or <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>31 demonstrated more effective cell\u2010mediated immunity, although some combinations of <jats:styled-content style=\"fixed-case\">rG<\/jats:styled-content>ro<jats:styled-content style=\"fixed-case\">EL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">rOMP<\/jats:styled-content>31 vaccines were more efficient than other divalent ones.<\/jats:p>","DOI":"10.1111\/apm.12778","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T13:12:36Z","timestamp":1511183556000},"page":"65-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of heat shock protein 60<scp>KD<\/scp> in combination with outer membrane proteins on immune response against <i>Brucella 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