{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:39:20Z","timestamp":1789767560727,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Visceral leishmaniasis (<jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content>) is the most severe form of leishmaniasis. Recent findings indicate that dendritic cells have a key role in the defense against the Leishmania parasite and that the activity of this cell may be modified by the eosinophil secretory protein eosinophil\u2010derived neurotoxin (<jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content>). We hypothesized that the interactions between dendritic cells and <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> might be of importance in the disease development. Cellular content of <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> was analyzed by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. The single\u2010nucleotide polymorphisms at positions 405, 416, and 1122 in the <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> gene were analyzed by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> with TaqMan<jats:sup>\u00ae<\/jats:sup> reagents. The study cohorts comprised 239 Sudanese subjects (65 healthy controls and 174 with <jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content>) and 300 healthy Swedish controls. The eosinophil content of <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> was lower in <jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content> as compared with controls (p &lt; 0.0001). The <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content>405 (G&gt;C) genotype distribution was similar among Swedish and Sudanese controls, whereas <jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content> subjects had a higher prevalence of the <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content>405\u2010<jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content> genotype (p &lt; 0.0001). The content of <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> in the eosinophils was closely linked to the <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content>405 polymorphism (p = 0.0002). Our findings suggest that the predisposition to acquire <jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content> is related to the genetic polymorphism of the <jats:styled-content style=\"fixed-case\">EDN<\/jats:styled-content> gene and the reduced production by the eosinophil of this gene product.<\/jats:p>","DOI":"10.1111\/apm.12780","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T17:54:26Z","timestamp":1511978066000},"page":"85-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The genetically determined production of the alarmin eosinophil\u2010derived neurotoxin is reduced in visceral leishmaniasis"],"prefix":"10.1111","volume":"126","author":[{"given":"Kristin","family":"Blom","sequence":"first","affiliation":[{"name":"Department of Medical Sciences Clinical Chemistry Uppsala University Uppsala 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