{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:14:47Z","timestamp":1696065287944},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T00:00:00Z","timestamp":1511136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002657","name":"Istanbul \u00dcniversitesi","doi-asserted-by":"publisher","award":["31970"],"award-info":[{"award-number":["31970"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Inexpensive, rapid, and reliable tests for detecting the presence and drug susceptibility of Mycobacterium tuberculosis<\/jats:italic> complex (MTBC<\/jats:styled-content>) are urgently needed to control the transmission of tuberculosis. In this study, we aimed to assess the accuracy and speed of the microscopic\u2010observation drug susceptibility (MODS<\/jats:styled-content>) assay in the identification of MTBC<\/jats:styled-content> and detection of multidrug resistance. Sputum samples from patients suspected to have tuberculosis were simultaneously tested with MODS<\/jats:styled-content> and conventional culture [L\u00f6wenstein\u2010Jensen (LJ<\/jats:styled-content>) culture, BACTEC MGIT<\/jats:styled-content>\u2122 960 (MGIT<\/jats:styled-content>) system], and drug susceptibility testing (MGIT<\/jats:styled-content> system) methods. A total of 331 sputum samples were analyzed. Sensitivity and specificity of MODS<\/jats:styled-content> assay for detection of MTBC<\/jats:styled-content> strains were 96% and 98.8%, respectively. MODS<\/jats:styled-content> assay detected multidrug resistant MTBC<\/jats:styled-content> isolates with 92.3% sensitivity and 96.6% specificity. Median time to culture positivity was similar for MGIT<\/jats:styled-content> (8\u00a0days) and MODS<\/jats:styled-content> culture (8\u00a0days), but was significantly longer with LJ<\/jats:styled-content> culture (20\u00a0days) (p\u00a0<\u00a00.0001 for both comparisons). Median time to availability of the susceptibility results was significantly (p\u00a0<\u00a00.0001) shorter with MODS<\/jats:styled-content> assay (8\u00a0days) than MGIT<\/jats:styled-content> system (20\u00a0days). In conclusion, MODS<\/jats:styled-content> is an inexpensive and rapid test with good performance characteristics for direct diagnosis of tuberculosis and detection of multidrug resistance.<\/jats:p>","DOI":"10.1111\/apm.12783","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T13:04:58Z","timestamp":1511183098000},"page":"38-44","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The value of microscopic\u2010observation drug susceptibility assay in the diagnosis of tuberculosis and detection of multidrug resistance"],"prefix":"10.1111","volume":"126","author":[{"given":"Den\u0130z","family":"Sertel \u015eelale","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Istanbul Faculty of Medicine Istanbul University Istanbul Turkey"},{"name":"Department of Medical Microbiology International School of Medicine Istanbul Medipol University Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meltem","family":"Uzun","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Istanbul Faculty of Medicine Istanbul University Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,20]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Global Tuberculosis Report 2016","author":"World Health Organization","year":"2016"},{"key":"e_1_2_6_3_1","unstructured":"World Health Organization.Global Tuberculosis Report 2015. 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