{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:47:19Z","timestamp":1762642039085},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T00:00:00Z","timestamp":1513123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005354","name":"Folktandv\u00e5rden, \u00d6rebro L\u00e4ns Landsting","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005354","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006736","name":"Universitetssjukhuset \u00d6rebro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006736","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p><jats:italic>Mycoplasma genitalium<\/jats:italic>, causing non\u2010gonococcal non\u2010chlamydial urethritis and associated with cervicitis, has developed antimicrobial resistance (AMR) to both the macrolide azithromycin (first\u2010line treatment) and the fluoroquinolone moxifloxacin (second\u2010line treatment). Our aim was to estimate the prevalence of resistance, based on genetic AMR determinants, to these antimicrobials in the <jats:italic>M. genitalium<\/jats:italic> population in two Swedish counties, \u00d6rebro and Halland, 2011\u20132015. In total, 672 <jats:italic>M. genitalium<\/jats:italic> positive urogenital samples were sequenced for 23S rRNA and <jats:italic>parC<\/jats:italic> gene mutations associated with macrolide and fluoroquinolone resistance, respectively. Of the samples, 18.6% and 3.2% in \u00d6rebro and 15.2% and 2.7% in Halland contained mutations associated with macrolide and fluoroquinolone resistance, respectively. The predominating resistance\u2010associated mutations in the 23S rRNA gene was A2059G (n = 39) in \u00d6rebro and A2058G (n = 13) and A2059G (n = 13) in Halland. The most prevalent possible resistance\u2010associated ParC amino acid alterations were S83I (n = 4) in \u00d6rebro and S83N (n = 2) in Halland. Resistance\u2010associated mutations to both macrolides and fluoroquinolones were found in 0.7% of samples. Our findings emphasize the need for routine AMR testing, at a minimum for macrolide resistance, of all <jats:italic>M. genitalium<\/jats:italic>\u2010positive samples and regular national and international surveillance of AMR in <jats:italic>M. genitalium<\/jats:italic>, to ensure effective patient management and rational antimicrobial use.<\/jats:p>","DOI":"10.1111\/apm.12792","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T06:35:31Z","timestamp":1513146931000},"page":"123-127","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Macrolide and fluoroquinolone resistance in <i>Mycoplasma genitalium<\/i> in two Swedish counties, 2011\u20132015"],"prefix":"10.1111","volume":"126","author":[{"given":"Ronza","family":"Hadad","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Clinical Microbiology Faculty of Medicine and Health WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections National Reference Laboratory for Sexually Transmitted Infections \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Golparian","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Clinical Microbiology Faculty of Medicine and Health WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections National Reference Laboratory for Sexually Transmitted Infections \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amaya C.","family":"Lagos","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Clinical Microbiology Faculty of Medicine and Health WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections National Reference Laboratory for Sexually Transmitted Infections \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Ljungberg","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Hospital of Halmstad Halmstad Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology &amp; Infection Control Hospital of Halmstad Halmstad Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rgen S.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control Sexually Transmitted Infections, Research and Development Statens Serum Institut Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Fredlund","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Clinical Microbiology Faculty of Medicine and Health WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections National Reference Laboratory for Sexually Transmitted Infections \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Clinical Microbiology Faculty of Medicine and Health WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections National Reference Laboratory for Sexually Transmitted Infections \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2016.268"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ312"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyv194"},{"key":"e_1_2_6_5_1","unstructured":"STI \u2010 behandlingsrekommendation: L\u00e4kemedelsverket2015;26(1).https:\/\/lakemedelsverket.se\/upload\/halso-och-sjukvard\/behandlingsrekommendationer\/Sexuellt_overforbara_bakteriella_infektioner_behandlingsrekommendation_webb.pdf(Accessed: August 28 2017). In Swedish."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13849"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462416648585"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462415618837"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/cid\/ciw719","article-title":"Azithromycin 1.5 g over 5 days compared to 1 g single dose in urethral Mycoplasma genitalium: impact on treatment outcome and resistance","volume":"64","author":"Read TR","year":"2017","journal-title":"Clin Infect Dis"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.05.011"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175763"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000246"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.3201\/eid2209.160446","article-title":"Fluoroquinolone\u2010resistant Mycoplasma genitalium, Southwestern France","volume":"22","author":"Le Roy C","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv136"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu217"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku164"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2106.142013"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462413502008"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01053-16"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.07.005"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3402\/ijch.v71i0.18203"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu1162"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"809","DOI":"10.3201\/eid2305.161745","article-title":"Increasing macrolide and fluoroquinolone resistance in Mycoplasma genitalium","volume":"23","author":"Murray GL","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061481"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1136\/sextrans-2016-052688","article-title":"Mycoplasma genitalium macrolide resistance in Stockholm, Sweden","volume":"93","author":"Bj\u00f6rnelius E","year":"2017","journal-title":"Sex Transm Infect"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.5.742"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00495-13"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.954"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix132"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-0781-7"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapm.12792","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.12792","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T23:42:42Z","timestamp":1695858162000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.12792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,13]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/apm.12792"],"URL":"https:\/\/doi.org\/10.1111\/apm.12792","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,13]]},"assertion":[{"value":"2017-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}