{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:07:27Z","timestamp":1781269647704,"version":"3.54.1"},"reference-count":68,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005725","name":"National Plan for Science, Technology and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:p>Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (<jats:styled-content style=\"fixed-case\">MAC<\/jats:styled-content>s). The <jats:styled-content style=\"fixed-case\">MAC<\/jats:styled-content> in each <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> of milk varied from 60 to 16\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\u00a0<jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de\u2010replicated into 80 unique isolates using rep\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis. Surprisingly, the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> sequence analysis of these strains revealed that more than one\u2010third of the collected milk samples contained strains that share maximum sequence similarities with well\u2010known pathogens, such as <jats:italic>Brucella<\/jats:italic>,<jats:italic> Bacillus anthracis<\/jats:italic>,<jats:italic> Listeria monocytogenes<\/jats:italic>, and <jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content>. Furthermore, many strains exhibit 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene similarity with opportunistic pathogens such as <jats:italic>Citrobacter freundii<\/jats:italic> and <jats:italic>Kytococcus schroeteri<\/jats:italic>. Many strains exhibit \u03b2\u2010hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.<\/jats:p>","DOI":"10.1111\/apm.12802","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T06:15:30Z","timestamp":1519712130000},"page":"215-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S <scp>rRNA<\/scp> gene similarity with well\u2010known pathogens found in camel milk samples of Riyadh region"],"prefix":"10.1111","volume":"126","author":[{"given":"Abdurahman H.","family":"Hirad","sequence":"first","affiliation":[{"name":"Botany and Microbiology Department College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javed","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Zoology Department College of Science 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