{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:07:44Z","timestamp":1778645264182,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T00:00:00Z","timestamp":1524614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>The immunodiagnostic tests for cystic echinococcosis (<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>) are mostly serological tests based on <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> that use hydatid cyst antigens for primary screening because of its simple preparation and availability. The challenge to develop new serological methods (as compared to those based on the hydatid cyst fluid antigens) to meet the gold standard remains. Appropriate sources of antigenic material are necessary for application to improve the efficacy of immunodiagnostic tests at a population level. In the current study, a fusion protein containing the coding sequence of antigen B2t and two sequences of <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>1 antigen with some modifications was reconstructed. Using bioinformatics tools, these sequences were joined together by applying the sequence of a rigid \u03b1\u2010helix\u2010forming linker to obtain an appropriate structure of a fusion protein. Synthetic recombinant fusion protein was expressed using <jats:styled-content style=\"fixed-case\">pET<\/jats:styled-content>28a as a vector and evaluated by indirect <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> test for sera from patients with hepatic <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> and other parasitic infections. The sensitivity of the fusion protein was lower (88.46%) than the available <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> kit (96.15%). However, the differences in sensitivity were not statistically significant as compared to the recombinant fusion peptide with the commercial kit (p\u00a0=\u00a00.269). The specificity of the recombinant fusion protein (95.45%) was not significantly lower than the commercial kit (96.59%; p\u00a0=\u00a01.000). Moreover, surprisingly there was no difference in the cross\u2010reactivity values of performance between the recombinant\u2010<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and commercial kit. The positive and negative predictive values of the recombinant antigen were achieved as 92% and 93.33%, respectively, while for the commercial kit, they were obtained as 94.33% and 97.70%, respectively. In conclusion, as an early evaluation of these antigens the performance of our recombinant fusion protein in <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> is relatively promising. Although, it seemed that this peptide with specific antigenic epitopes might be more appropriate for the serological evaluation of <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> by use of bioinformatics tools, our findings showed that cross\u2010reactions and a negative reaction could occur in clinical performance. This fusion protein may have utility for diagnosis in humans, but further evaluation is needed using the <jats:styled-content style=\"fixed-case\">WHO<\/jats:styled-content> ultrasound classification for <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/apm.12838","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T05:05:21Z","timestamp":1524719121000},"page":"428-439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Design and construction of a new recombinant fusion protein (2b2t+<scp>EPC<\/scp>1) and its assessment for serodiagnosis of cystic echinococcosis"],"prefix":"10.1111","volume":"126","author":[{"given":"Saeid","family":"Fathi","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine University of Tehran Tehran 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