{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:55:30Z","timestamp":1790470530241,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T00:00:00Z","timestamp":1540425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/18990\u20102"],"award-info":[{"award-number":["2015\/18990\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>This study aimed to investigate the antimicrobial resistance profile to quinolones, the presence of quinolone\u2010resistant determinants and the plasmid replicon typing in environmental <jats:italic>Achromobacter<\/jats:italic> sp. isolated from Brazil. Soil and water samples were used for bacterial isolation. The antimicrobial susceptibility testing was performed by minimum inhibitory concentration method. The detection of mutations in the quinolone resistance\u2010determining regions (QRDR) genes, the presence of plasmid\u2010mediated quinolone resistance (PMQR) genes, and plasmid replicons were performed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. A total of 16 isolates was obtained from different cultures, cities, and states of Brazil. All isolates were non\u2010susceptible to ciprofloxacin, norfloxacin, and levofloxacin. Some mutations in <jats:styled-content style=\"fixed-case\">QRDR<\/jats:styled-content> genes were found, including Gln\u201083\u2010Leu and Asp\u201087\u2010Asn in the <jats:italic>gyr<\/jats:italic>A and Gln\u201080\u2010Ile and Asp\u201084\u2010Ala in the <jats:italic>par<\/jats:italic>C. Different <jats:styled-content style=\"fixed-case\">PMQR<\/jats:styled-content> genes were detected, such as <jats:italic>qnr<\/jats:italic>A, <jats:italic>qnr<\/jats:italic>B, <jats:italic>qnr<\/jats:italic>S, <jats:italic>oqx<\/jats:italic>A, and <jats:italic>oqx<\/jats:italic>B. Three different plasmid families were detected, being most presented the ColE\u2010<jats:italic>like<\/jats:italic>, followed by Inc<jats:styled-content style=\"fixed-case\">FIB<\/jats:styled-content> and IncA\/C. The presence of different <jats:styled-content style=\"fixed-case\">PMQR<\/jats:styled-content> genes and plasmids in the isolates of the present study shows that environmental bacteria can act as reservoir of important genes of resistance to fluoroquinolones, which is of great concern, due to the potential of horizontal dissemination of these genes. Besides that, there are no studies reporting these results in <jats:italic>Achromobacter<\/jats:italic> sp. isolates.<\/jats:p>","DOI":"10.1111\/apm.12896","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T09:54:12Z","timestamp":1540461252000},"page":"864-869","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Replicon typing of plasmids in environmental <i>Achromobacter<\/i> sp. producing quinolone\u2010resistant determinants"],"prefix":"10.1111","volume":"126","author":[{"given":"Jo\u00e3o Pedro Rueda","family":"Furlan","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo (USP) Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo Garcia","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo (USP) Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inara Fernanda Lage","family":"Gallo","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo (USP) Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana Guedes","family":"Stehling","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo (USP) Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1396929"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201406-288FR"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00138"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-016-0736-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg207"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2004.01.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/428052"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.3.2221-2225.2006"},{"key":"e_1_2_8_10_1","unstructured":"CLSI.Performance standards for antimicrobial susceptibility testing: twenty\u2010fifth informational supplement. 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