{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T06:51:49Z","timestamp":1772866309749,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T00:00:00Z","timestamp":1540425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>The aim of the study was to validate whether rapid antimicrobial susceptibility testing (AST) by very early reading of disc diffusion tests would provide reliable results in daily routine work. A total of 264 positive blood culture bottles were examined, of which 178 were examined as part of the daily routine workflow. <jats:italic>Enterobacterales<\/jats:italic> were evaluated for resistance to cefotaxime, ceftazidime, gentamicin and ampicillin. <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S.\u00a0aureus<\/jats:italic>) was tested for resistance to cefoxitin as a marker of methicillin\u2010resistant <jats:italic>S.\u00a0aureus<\/jats:italic> (MRSA). The zones were read after 3\u00a0h, and if there was insufficient growth, 30 and 60\u00a0min later. The results were compared to standard overnight AST. For ampicillin, gentamicin and cefoxitin, there were no errors. For cefotaxime, there were three minor (1.5%), three major (1.5%) and no very major errors. For ceftazidime, there were 13 minor (6.5%) errors only. With the exception of one minor error, all errors were ESBL\u2010A\u2010 or AmpC\u2010producing isolates where rapid AST showed a higher degree of resistance than standard AST. This low\u2010cost method may contribute to early effective antibacterial treatment by providing reliable results of AST within 3\u20134\u00a0h. Special breakpoints for early reading are a prerequisite.<\/jats:p>","DOI":"10.1111\/apm.12897","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T09:54:12Z","timestamp":1540461252000},"page":"870-876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid antimicrobial susceptibility testing of positive blood cultures by direct inoculation and reading of disc diffusion tests after 3\u20134 hours"],"prefix":"10.1111","volume":"126","author":[{"given":"Einar Tollaksen","family":"Weme","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Vestre Viken Hospital Trust Drammen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1128\/jcm.32.7.1757-1762.1994","article-title":"Clinical impact of rapid in\u00a0vitro susceptibility testing and bacterial identification","volume":"32","author":"Doern GV","year":"1994","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.06.006"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1128\/jcm.20.3.473-477.1984","article-title":"Rapid antimicrobial susceptibility testing of isolates from blood cultures by direct inoculation and early reading of disk diffusion tests","volume":"20","author":"Coyle MB","year":"1984","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01679-13"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","unstructured":"JonassonE.Proposed breakpoints for rapid antimicrobial susceptibility testing with disc diffusion tests direct from positive blood cultures for Escherichia coli Klebsiella pneumoniae Staphylococcus aureus and Streptococcus pneumoniae. 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Available from:http:\/\/www.eucast.org\/clinical_breakpoints\/[updated 2018 May 16; cited 2018 Jul 18]."},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1128\/JCM.38.6.2429-2433.2000","article-title":"Rapid identification of methicillin\u2010resistant Staphylococcus aureus and simultaneous species confirmation using real\u2010time fluorescence PCR","volume":"38","author":"Reischl U","year":"2000","journal-title":"J Clin Microbiol"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapm.12897","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.12897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T00:38:56Z","timestamp":1694824736000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.12897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,25]]},"references-count":13,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/apm.12897"],"URL":"https:\/\/doi.org\/10.1111\/apm.12897","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,25]]},"assertion":[{"value":"2018-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}