{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:44:02Z","timestamp":1765295042537},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T00:00:00Z","timestamp":1551225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>The aim of this study was to assess the role of cytology, human papilloma virus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and human papilloma virus messenger <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">HPV mRNA<\/jats:styled-content>) assays in detecting cervical intraepithelial neoplasia grade 2+ (CNi 2+) (recurrences\/persistence) during the follow\u2010up of women after treatment of cervical intraepithelial lesion. This cross\u2010sectional study was performed among 43 women treated for cervical intraepithelial neoplasia (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>) between January 2014 and January 2017 at the Department of Obstetrics and Gynecology of Spedali Civili's Hospital, Brescia, Italy. Pap smear and cervical samples for <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> tests were collected during the follow\u2010up visit. Furthermore, colposcopy was always performed in order to find out the persistence\/recurrence of the disease. A cervical biopsy was collected when necessary. Cervical samples obtained were tested for <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> using the <jats:styled-content style=\"fixed-case\">INNO<\/jats:styled-content>\u2010LiPa <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> assay and for <jats:styled-content style=\"fixed-case\">HPV mRNA<\/jats:styled-content> using the <jats:styled-content style=\"fixed-case\">APTIMA<\/jats:styled-content> assay. The mean age of enrolled women was 42.5\u00a0years. Among the treated patients, more than 50% of women revealed the absence of high risk <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HPV mRNA<\/jats:styled-content>. We found the persistence of the disease cervical intraepithelial neoplasia grade 2 (CIN 2) only in one woman. The sensitivity of cytology, <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HPV mRNA<\/jats:styled-content> in detecting disease was satisfactory (100%), while the specificity was quite different for the three tests: 64.2, 52.4 and 78.9%, respectively. The <jats:styled-content style=\"fixed-case\">HPV mRNA<\/jats:styled-content> test has higher specificity with respect to cytology and <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content>, avoiding the referral to unnecessary colposcopy with an improvement of costs\/benefits for healthcare system. However, given the small size sample, this study should be considered as a pilot for future larger studies.<\/jats:p>","DOI":"10.1111\/apm.12931","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T07:34:38Z","timestamp":1551339278000},"page":"196-201","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Role of <scp>HPV DNA<\/scp>,<scp> HPV mRNA<\/scp> and cytology in the follow\u2010up of women treated for cervical dysplasia"],"prefix":"10.1111","volume":"127","author":[{"given":"Giancarlo","family":"Tisi","sequence":"first","affiliation":[{"name":"Gynecologic and Obstetrical Division University of Brescia\u2010Spedali Civili di Brescia Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Gargiulo","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine Institute of Microbiology University of 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