{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:14:40Z","timestamp":1790338480449,"version":"4.1.0"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Rapid identification of <jats:italic>Candida<\/jats:italic> species is important for appropriate antifungal therapy of fungemia. The matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content>) system is a useful tool to identify bacteria and yeasts. In this study, we evaluated the feasibility of identifying yeasts after a short\u2010term incubation on a solid medium. We tested 24 strains of eight <jats:italic>Candida<\/jats:italic> species. Blood culture bottles were spiked with a calibrated suspension of each <jats:italic>Candida<\/jats:italic> strain. Three different culture media, two types of blood culture bottles and three different incubation time points were tested. A multivariable random\u2010effects logistic regression analysis was performed for determining factors independently associated with a successful <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content> identification. One\u2010hundred and thirty\u2010one out of 432 <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content> analyses (30%) exhibited a score\u00a0\u2265\u00a01.7. The performance of the technique varied across <jats:italic>Candida<\/jats:italic> species. Factors associated with a successful identification were the use of a chromogenic Candida medium and the time points 4 and 5\u00a0h. Using the factors \u2018chromogenic Candida medium\u2019 and time point 5\u00a0h the global performance of identification reached 60% and a mean <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> score of 1.78. Identifying yeasts after a short\u2010term incubation on a solid medium seems possible, especially when using a chromogenic Candida medium and respecting at least 5\u00a0h of incubation. This assay was a first step and needs to be completed using more strains, various chromogenic Candida medium and maybe also testing a longer culture time such as 6\u00a0h.<\/jats:p>","DOI":"10.1111\/apm.12936","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T06:27:04Z","timestamp":1551162424000},"page":"217-221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rapid identification of <i>Candida<\/i> sp. by <scp>MALDI<\/scp>\u2010<scp>TOF<\/scp> mass spectrometry subsequent to short\u2010term incubation on a solid medium"],"prefix":"10.1111","volume":"127","author":[{"given":"Anne\u2010Pauline","family":"Bellanger","sequence":"first","affiliation":[{"name":"Parasitology Mycology Department Jean Minjoz University Hospital Besancon France"},{"name":"Chrono\u2010Environment UMR\/CNRS 6249 University of Bourgogne Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houssein","family":"Gbaguidi\u2010Haore","sequence":"additional","affiliation":[{"name":"Infection Control Department Jean Minjoz University Hospital Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Liapis","sequence":"additional","affiliation":[{"name":"Bacteriology Department Jean Minjoz University Hospital Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeline","family":"Scherer","sequence":"additional","affiliation":[{"name":"Parasitology Mycology Department Jean Minjoz University Hospital Besancon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"Parasitology Mycology Department Jean Minjoz University Hospital Besancon France"},{"name":"Chrono\u2010Environment UMR\/CNRS 6249 University of Bourgogne Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3408-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.07.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw038"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03600-14"},{"key":"e_1_2_8_6_1","first-page":"245","article-title":"Direct identification of microorganisms from positive blood cultures using the lysis\u2010filtration technique and matrix assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS): a multicenter study","volume":"38","author":"Farina C","year":"2015","journal-title":"New 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