{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T19:30:03Z","timestamp":1783711803692,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T00:00:00Z","timestamp":1563494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:p>Microfibrillar\u2010associated protein 4 (MFAP4) is a non\u2010structural matrix protein with cell regulatory activities and a potential as seromarker for fibrosis. We aimed to study the occurrence of MFAP4 in the synovial membrane from patients with rheumatoid arthritis (RA) vs osteoarthritis (OA). Formaldehyde\u2010fixed synovial tissue sections, from patients with RA (N\u00a0=\u00a06) and OA (N\u00a0=\u00a06) undergoing total hip arthroplasty, were deparaffinized and immunostained with monoclonal antibodies against MFAP4. Elastin was detected using ElastiKit. MFAP4 in serum (sMFAP4) and synovial fluid was measured by an immunoassay. MFAP4 was present in synovial biopsies from both RA and OA patients, particularly prominent in deep arterioles where it colocalized with elastin. Notably however, MFAP4 was absent from the internal elastic lamina in RA arterioles irrespective of disease duration and synovitis activity, while present although with irregular staining patterns in OA specimens. sMFAP4 was increased in RA and OA serum vs healthy controls: median (interquartile range) 29.8 (25.3\u201339.1) and 25.5\u00a0U\/L (18.1\u201343.3) vs 17.7\u00a0U\/L (13.7\u201321.2), p\u00a0=\u00a00.006 and p\u00a0=\u00a00.02, respectively The concentration of synovial fluid was lower than in serum in both RA and OA. These findings may suggest that MFAP4 is involved in adaptive vessel wall remodeling associated with chronic joint disease.<\/jats:p>","DOI":"10.1111\/apm.12974","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T14:17:31Z","timestamp":1561472251000},"page":"588-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Site\u2010specific absence of microfibrillar\u2010associated protein 4 (MFAP4) from the internal elastic membrane of arterioles in the rheumatoid arthritis synovial membrane: an immunohistochemical study in patients with advanced rheumatoid arthritis versus osteoarthritis"],"prefix":"10.1111","volume":"127","author":[{"given":"Anne Friesgaard","family":"Christensen","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Lillebaelt Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grith Lykke","family":"Sorensen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Junker","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Revald","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Lillebaelt Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Varnum","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Lillebaelt Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saida Farah","family":"Issa","sequence":"additional","affiliation":[{"name":"The Rheumatology Research Unit, Department of Rheumatology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Junker","sequence":"additional","affiliation":[{"name":"The Rheumatology Research Unit, Department of Rheumatology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flemming Brandt","family":"Sorensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Lillebaelt Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1004965"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.200"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20878"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211735"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12413"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200005)43:5<953::AID-ANR1>3.0.CO;2-Q"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3280"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140418"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22764"},{"key":"e_1_2_5_11_1","first-page":"41","article-title":"MFAP4: A candidate 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