{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:13:56Z","timestamp":1763018036050},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:00:00Z","timestamp":1565654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>Sexually transmitted infections (STIs) remain major public health problems globally. Appropriate laboratory diagnosis of STIs is rare in Ukraine. We investigated the prevalence of <jats:italic>Chlamydia trachomatis<\/jats:italic> (CT), <jats:italic>Neisseria gonorrhoeae<\/jats:italic> (NG) and <jats:italic>Trichomonas vaginalis<\/jats:italic> (TV) using the US FDA\u2010approved Aptima Combo 2 and Aptima TV assays and compared the results with the conventional routine diagnostic tests (CDTs) in Ukraine. Urogenital swabs from consecutive mostly symptomatic females (n\u00a0=\u00a0296) and males (n\u00a0=\u00a0159) were examined. The prevalences were as follows: 10% (n\u00a0=\u00a047) of TV, 5.3% (n\u00a0=\u00a024) of CT and 1.5% (n\u00a0=\u00a07) of NG. The specificity of some CDTs was high, for example, 100% for NG culture, TV IgG ELISA, CT IgM ELISA and CT microscopy, but lower for other CDTs, that is, from 44% to 99.8%. The sensitivity of all CDTs was suboptimal, that is, 71% (n\u00a0=\u00a05) for NG microscopy, 57% (n\u00a0=\u00a04) for NG culture, 53% (n\u00a0=\u00a08) for CT IgG ELISA, 33% (n\u00a0=\u00a01) for TV IgG ELISA, 28% (n\u00a0=\u00a013) for TV microscopy, 25% (n\u00a0=\u00a01) for CT IgA ELISA, 20% (n\u00a0=\u00a03) for CT IgM ELISA and 0% (n\u00a0=\u00a00) for CT microscopy. The prevalences of particularly TV and CT were high, but substantial also for NG, in Ternopil, Ukraine. The sensitivities of all CDTs were low, and widespread implementation of validated, quality\u2010assured and cost\u2010effective molecular diagnostic STI tests in Ukraine is imperative.<\/jats:p>","DOI":"10.1111\/apm.12975","type":"journal-article","created":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T14:04:05Z","timestamp":1561125845000},"page":"627-634","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["High prevalence of <i>Chlamydia trachomatis, Neisseria gonorrhoeae<\/i> and particularly <i>Trichomonas vaginalis<\/i> diagnosed using US FDA\u2010approved Aptima molecular tests and evaluation of conventional routine diagnostic tests in Ternopil, Ukraine"],"prefix":"10.1111","volume":"127","author":[{"given":"Iryna","family":"Boiko","sequence":"first","affiliation":[{"name":"Clinical Laboratory Department Ternopil Regional Clinical Dermatovenerologic Dispensary Ternopil Ukraine"},{"name":"Department of Functional and Laboratory Diagnostics I. Horbachevsky Ternopil State Medical University Ternopil Ukraine"},{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Golparian","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inna","family":"Krynytska","sequence":"additional","affiliation":[{"name":"Department of Functional and Laboratory Diagnostics I. Horbachevsky Ternopil State Medical University Ternopil Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,13]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"World Health Organization.Report on global sexually transmitted infection surveillance 2018.Geneva:World Health Organization;2018. Available at:https:\/\/www.who.int\/reproductivehealth\/publications\/stis-surveillance-2018\/en\/(Accessed February 12 2019)."},{"key":"e_1_2_6_3_1","unstructured":"European Centre for Disease Prevention and Control.Surveillance Atlas of Infectious Diseases. Available at:http:\/\/atlas.ecdc.europa.eu\/public\/index(Accessed February 12 2019)."},{"key":"e_1_2_6_4_1","unstructured":"Centralized information system for infectious diseases (CISID)\/Sexually transmitted infections (STI).Incidence rates (per 100 000 population). 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The Order from 04.07.2004 N. 286. Available at:http:\/\/medstandart.net\/browse\/1254(Accessed: February 12 2019). [In Ukrainian]."},{"key":"e_1_2_6_30_1","unstructured":"The Ministry of Health of Ukraine.[Regarding Clinical Protocols of Medical Aid to Patients with Dermatovenereological Diseases]. The Order from 08.05.2009 N 312 with changes and additions made 04.07.2016. Available at:http:\/\/old.moz.gov.ua\/ua\/portal\/dn_20090508_312.html(Accessed: February 12 2019). 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