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A total of 536 swabs (134 of patients and 402 of <jats:styled-content style=\"fixed-case\">ICU<\/jats:styled-content> environment) were collected and analyzed to detect <jats:italic>S.\u00a0aureus<\/jats:italic>. The antimicrobial susceptibility of the isolates was determined by disk diffusion test, and the detection of the <jats:italic>mec<\/jats:italic>A and virulence factors genes was performed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, in addition to <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content><jats:italic>mec<\/jats:italic> typing. The genetic similarity of the isolates was determined by <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>. <jats:italic>Staphylococcus aureus<\/jats:italic> was isolated in 12.7% of the swabs. The prevalence of colonization was 13.4% in patients and 12.4% in the environmental samples. The multidrug resistance was determined in 82.4% of the isolates. The prevalence of methicillin\u2010resistant <jats:italic>S.\u00a0aureus<\/jats:italic> was 20.6%, with 50.0% classified as <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content><jats:italic>mec <\/jats:italic><jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>. The intermediate resistance to vancomycin was detected in 5.9% and 4.4% of the isolates obtained from patients and environment, respectively. Identical isolates obtained from different patients and sources were grouped into several clusters. The results showed dissemination of multidrug\u2010resistant strains between patients and fomites and the persistence of <jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">VISA<\/jats:styled-content> isolates in the <jats:styled-content style=\"fixed-case\">ICU<\/jats:styled-content> environment.<\/jats:p>","DOI":"10.1111\/apm.12989","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T06:53:40Z","timestamp":1565679220000},"page":"717-726","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Methicillin\u2010resistant and vancomycin\u2010intermediate <i>Staphylococcus aureus<\/i> colonizing patients and intensive care unit environment: virulence profile and genetic variability"],"prefix":"10.1111","volume":"127","author":[{"given":"J\u00e9ssica O.","family":"Veloso","sequence":"first","affiliation":[{"name":"Instituto Carlos Chagas Funda\u00e7\u00e3o Oswaldo Cruz Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Lamaro\u2010Cardoso","sequence":"additional","affiliation":[{"name":"Departamento de Bioci\u00eancias e Tecnologia Instituto de Patologia Tropical e Sa\u00fade P\u00fablica Universidade Federal de Goi\u00e1s Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorrane S.","family":"Neves","sequence":"additional","affiliation":[{"name":"Departamento de Bioci\u00eancias e Tecnologia Instituto de Patologia Tropical e Sa\u00fade P\u00fablica Universidade Federal de Goi\u00e1s Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizandra F. 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