{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:09:54Z","timestamp":1791846594325,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T00:00:00Z","timestamp":1578009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["[no. 3\/1\/3\/WL\/JRF\u20102011\/HRD\u2010129 (41937)]"],"award-info":[{"award-number":["[no. 3\/1\/3\/WL\/JRF\u20102011\/HRD\u2010129 (41937)]"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Rapid dissemination of carbapenem\u2010resistant Gram\u2010negative bacteria (CRGNB) is a global threat. Quercetin is known for its antimicrobial activity. In this study, carbapenemase and efflux pump inhibitory activities of quercetin were demonstrated against carbapenem\u2010resistant <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Acinetobacter baumannii<\/jats:italic>. Further, molecular docking was performed to elucidate molecular mechanisms of such inhibition. CRGNB, expressing one of the carbapenemases, demonstrated significant inhibition of carbapenemase activity when pre\u2010incubated with 64\u00a0\u00b5g\/ml quercetin. Moreover, <jats:italic>acrB<\/jats:italic> overexpressing enterobacterial isolates exhibited significant inhibition of efflux activity upon quercetin treatment. Molecular docking studies revealed stability of quercetin\u2010carbapenemase complexes. (i) Virtual superimposition of quercetin onto meropenem, (ii) proximity of quercetin to attacking nucleophile and (iii) involvement of same amino acids that stabilize both meropenem and quercetin \u2013 indicated competition between quercetin and meropenem for ligand binding. Although quercetin and PA\u03b2N, a standard efflux pump inhibitor, docked at both central cavity and periplasmic drug binding sites of AcrB, they did not virtually superimpose on each other. However, sufficient release of Gibb's free energy and involvement of same set of amino acids in PA\u03b2N and quercetin stability predicted quercetin's efflux pump inhibitory potential. Hence, quercetin could be potential adjuvant therapeutics for CRGNB\u2010mediated infections.<\/jats:p>","DOI":"10.1111\/apm.13015","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T12:06:33Z","timestamp":1574424393000},"page":"251-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Quercetin inhibits carbapenemase and efflux pump activities among carbapenem\u2010resistant Gram\u2010negative bacteria"],"prefix":"10.1111","volume":"128","author":[{"given":"Arijit","family":"Pal","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Medical Biotechnology Calcutta School of Tropical Medicine Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anusri","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Biotechnology Calcutta School of Tropical Medicine Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,3]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization: Geneva.Guidelines for the prevention and control of carbapenem\u2010resistant Enterobacteriaceae Acinetobacter baumannii and Pseudomonas aeruginosa in health care facilities;2017."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-07"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw282"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3244-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00042-16"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14388"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11054.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-024-1045-7_4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.7b00225"},{"key":"e_1_2_8_11_1","first-page":"247","article-title":"In search of natural substrates and inhibitors of MDR pumps","volume":"3","author":"Lewis K","year":"2001","journal-title":"J Mol Microbiol Biotechnol"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2016.1232402"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124814"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_716_17"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/783540"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01591-05"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.05.021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01011"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2016.06.001"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr331"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40360-016-0083-8"},{"key":"e_1_2_8_23_1","first-page":"2427","article-title":"Insilicomodeling and docking studies of New Delhi Metallo\u2010Beta\u2010Lactamase\u20101 (super bug)","volume":"3","author":"Ganugapati J","year":"2011","journal-title":"Int J Eng Sc Tech"},{"key":"e_1_2_8_24_1","first-page":"328","article-title":"Inhibition of NDM\u20101 in superbugs by flavonoids an insilico approach","volume":"3","author":"Padmavathi M","year":"2012","journal-title":"J Adv Bioinfo Appl Res"},{"key":"e_1_2_8_25_1","first-page":"97","article-title":"Molecular docking analysis of predominant phytoligands against New Delhi Metallo\u2010Beta\u2010Lactamase\u20101 harboring Escherichia coli","volume":"5","author":"Thakur P","year":"2014","journal-title":"J Adv Bioinfo Appl Res"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2014.974211"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.212"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.13015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T07:40:20Z","timestamp":1693986020000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,3]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1111\/apm.13015"],"URL":"https:\/\/doi.org\/10.1111\/apm.13015","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,3]]},"assertion":[{"value":"2019-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}