{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:37:06Z","timestamp":1787668626664,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic> infection is the most common bacterial sexually transmitted infection globally, and nucleic acid amplification tests (NAATs) are recommended for highly sensitive and specific diagnosis. In early 2019, the Finnish new variant of <jats:italic>Chlamydia trachomatis<\/jats:italic> (FI\u2010nvCT) was identified. The FI\u2010nvCT has a C1515T mutation in the 23S rRNA gene, making it escaping detection in the Aptima Combo 2 (AC2; Hologic) NAAT, and the FI\u2010nvCT has been subsequently reported in Sweden and Norway. In the present study, we investigated the presence of the FI\u2010nvCT and other AC2 diagnostic\u2010escape CT mutants in July\u2013September 2019 in Denmark. The FI\u2010nvCT was present but rare in Denmark. However, another AC2 diagnostic\u2010escape CT mutant (with a 23S rRNA G1523A mutation) was found to be widespread across Denmark, accounting for 95% (76\/80) of AC2 diagnostic\u2010escape nvCT samples from five Danish CT\u2010diagnostic laboratories. This nvCT\u2010G1523A has previously only been detected in one single sample in the United Kingdom and Norway, respectively. It is vital to monitor the continued stability of the NAAT targets in local, national and international settings and monitor as well as appropriately analyse incidence, unexplained shifts in diagnostics rates and\/or annual collections of samples diagnosed as negative\/equivocal using NAATs with different target(s). Furthermore, diagnostic CT NAATs with dual target sequences are crucial, and fortunately, an updated Hologic AC2 assay including one additional target sequence is in advanced development.<\/jats:p>","DOI":"10.1111\/apm.13043","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T12:46:59Z","timestamp":1584967619000},"page":"440-444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A <i>Chlamydia trachomatis<\/i> 23S rRNA G1523A variant escaping detection in the Aptima Combo 2 assay (Hologic) was widespread across Denmark in July\u2013September 2019"],"prefix":"10.1111","volume":"128","author":[{"given":"Ronza","family":"Hadad","sequence":"first","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for STIs Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen Skov","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department for Bacteria, Parasites and Fungi, Infectious Diseases Preparedness Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Westh","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Hvidovre University Hospital Hvidovre Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Gr\u00f8nb\u00e6k","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Hvidovre University Hospital Hvidovre Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lasse Jessen","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department for Bacteria, Parasites and Fungi, Infectious Diseases Preparedness Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias M\u00fcller","family":"Vang","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Aalborg University Hospital Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenette Sandborg","family":"Weinreich","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Aalborg University Hospital Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne N.","family":"Skov","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Kj\u00f8lseth","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Vejle University Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birte","family":"Kolmos","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Vejle University Hospital Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other STIs National Reference Laboratory for STIs Department of Laboratory Medicine, Microbiology Faculty of Medicine and Health \u00d6rebro University \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Department for Bacteria, Parasites and Fungi, Infectious Diseases Preparedness Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.18.228486"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462415618837"},{"issue":"03","key":"e_1_2_6_4_1","first-page":"1","article-title":"Centers for Disease Control and Prevention. 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