{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T20:39:19Z","timestamp":1694119159089},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p>The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an <jats:italic>in vitro<\/jats:italic> experiment. Biofilms of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH\u2010adjusted and non\u2010pH\u2010adjusted media solutions were applied as control groups. Results showed complete eradication of <jats:italic>P. aeruginosa<\/jats:italic> biofilm with 0.50% v\/v acetic acid. Biofilm of <jats:italic>S. aureus<\/jats:italic> was eradicated with 1.25% v\/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/apm.13044","type":"journal-article","created":{"date-parts":[[2020,4,11]],"date-time":"2020-04-11T17:58:45Z","timestamp":1586627925000},"page":"445-450","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study"],"prefix":"10.1111","volume":"128","author":[{"given":"Mathilde","family":"Kjeldsen","sequence":"first","affiliation":[{"name":"Costerton Biofilm Center University of Copenhagen Copenhagen N Denmark"},{"name":"Department of Otorhinolaryngology and Maxillofacial Surgery Zealand University Hospital K\u00f8ge 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